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DNA methylation and histone deacetylation in neural stem cells

ABSTRACT: Neurosphere cultures were established from individually isolated E 14.5 forebrains of mouse embryos that carry a bcl2 transgene. Single-cell suspensions were prepared, seeded at 1x105 cells/ml and treated for two days with 150 nM Trichostatin A (TSA; histone deacetylase inhibitor), 500 nM 5-Aza-2’-deoxycytidine (AzaC; demethylating agent) or both compounds, or left untreated. Two independent experiments were performed. Keywords: other

ORGANISM(S): Mus musculus

PROVIDER: GSE587 | GEO | 2004/01/04

SECONDARY ACCESSION(S): PRJNA85369

REPOSITORIES: GEO

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Transcription profiling of murine neurosphere cultures carrying a Bcl2 transgene and treated with histone deacetylase inhibitor Trichostatin A and demethylating agent AzaC to study DNA methylation and histone deacetylation in neural stem cells.

Project description:Neurosphere cultures were established from individually isolated E 14.5 forebrains of mouse embryos that carry a bcl2 transgene. Single-cell suspensions were prepared, seeded at 1x105 cells/ml and treated for two days with 150 nM Trichostatin A (TSA; histone deacetylase inhibitor), 500 nM 5-Aza-2-deoxycytidine (AzaC; demethylating agent) or both compounds, or left untreated. Two independent experiments were performed.

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2022-09-19 | PXD031455 | Pride

Project description:To identify hypermethylated genes in induced pluripotent stem cell lines, human embryonic stem cell lines, and non-nucleofected CD34+ CB cells, indicated cell lines were treated with 5-aza-2′-deoxycytidine (DAC) or trichostatin A (TSA), and drug-induced changes in gene expression vs control treatment (PBS) were identified using Agilent two-color arrays, using the experimental methods of Ohm et al. (Cancer Res 70:7662-7673 2010).

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