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MiRNA profiling of human prostate cancer cell lines treated with 5azadC and TSA to investigate epigenetic modifications

ABSTRACT: miRNA expression profiling of prostate cancer cell lines, PC-3, DU145, LAPC-4, VCaP, LNCaP, 22rv1, and normal prostate epithelial cells, PrECs, was done after treating the cells with DNA demethylating agent 5-aza-2'-deoxycytidine (5azadC; Sigma-Aldrich, St. Louis, MO) and histone deacetylase inhibitor trichostatin A (TSA; Sigma-Aldrich). These treatments relieve epigenetic modifications, and thus reveal potentially epigenetically silenced miRNAs amongst the miRNAs with increased expression after the treatments.

ORGANISM(S): Homo sapiens

DISEASE(S): prostate carcinoma

SUBMITTER: Hanna Rauhala

PROVIDER: E-MEXP-2313 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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miR-193b is an epigenetically regulated putative tumor suppressor in prostate cancer.

Rauhala Hanna E HE Jalava Sanni E SE Isotalo Jarkko J Bracken Hazel H Lehmusvaara Saara S Tammela Teuvo L J TL Oja Hannu H Visakorpi Tapio T

International journal of cancer 20100901 6

miRNAs have proven to be key regulators of gene expression and are differentially expressed in various diseases, including cancer. Our aim was to identify epigenetically dysregulated genes in prostate cancer. We performed miRNA expression profiling after relieving epigenetic modifications in 6 prostate cancer cell lines and nonmalignant prostate epithelial cells. Thirty-eight miRNAs showed increased expression in any prostate cancer cell line after 5-aza-2'-deoxycytidine (5azadC) and trichostati ...[more]

PMID: 20073067

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