Highly sensitive amplicon-based transcript quantification by semi-conductor sequencing
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ABSTRACT: We have adapted a commercial assay that employs mRNA quantification based on the frequency of PCR amplicons determined by next-generation to a high-throughput semi-conductor sequencing platform (Ion-Torrent Proton). We show parallel amplification of pathway derived transcript sets/genes in 12 reference RNA samples followed by sequence-based quantification covering a dynamic range of five orders of magnitude with low technical variation.
ORGANISM(S): Homo sapiens
PROVIDER: GSE58737 | GEO | 2014/07/15
SECONDARY ACCESSION(S): PRJNA253305
REPOSITORIES: GEO
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