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HPL-2 in GW638 (met-2 set-25) MxEmb

ABSTRACT: ChIP-chip of HPL-2 in met-2 set-25 C. elegans mixed-stage embryo

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE58761 | GEO | 2014/10/11

SECONDARY ACCESSION(S): PRJNA253400

REPOSITORIES: GEO

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HPL-2 in GW638 (met-2 set-25) MxEmb

Project description:ChIP-chip of HPL-2 in met-2 set-25 C. elegans mixed-stage embryo EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: GW638; Developmental Stage: Mixed-stage Embryo; Genotype: met-2(n4256) set-25(n5021); Sex: population predominantly Hermaphrodites perhaps with some Males; NUMBER OF REPLICATES: 2; EXPERIMENTAL FACTORS: temperature 20

2014-10-11 | E-GEOD-58761 | biostudies-arrayexpress

HPL-2 in PFR40 (hpl-2) early embryo

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LMN-1-DamID in N2 and set-25(n5021) met-2(n4256) mutant early C. elegans embryos

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HPL-2 in PFR40 (hpl-2) early embryo

Project description:ChIP-chip of HPL-2 in hpl-2 C. elegans early embryo EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: PFR40; Developmental Stage: Early Embryo; Genotype: hpl-2(tm1489); Sex: population predominantly Hermaphrodites perhaps with some Males; NUMBER OF REPLICATES: 1; EXPERIMENTAL FACTORS: temperature 20

2014-10-11 | E-GEOD-58763 | biostudies-arrayexpress

HPL-2 in N2 (wild-type) early embryo

Project description:ChIP-chip of HPL-2 in N2 C. elegans early embryo

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LMN-1-DamID in N2 and set-25(n5021) met-2(n4256) mutant early C. elegans embryos

Project description:We asked if the perinuclear position of chromosome arms in C. elegans depends on the histone methyltransferases MET-2 and SET-25. To this end, we performed LMN-1-DamID in wild-type (N2) and mutant (set-25 met-2) strains. LMN-1-DamID signal on chromosome arms was significantly reduced in the mutant. Three biological replicas were performed for each genotype. For one replica dyes were swapped. For each replica methylated DNA amplified from a strain expressing LMN-1-Dam was competitively hybridized against DNA amplified from a strain expressing GFP-Dam.

2012-09-04 | E-GEOD-37226 | biostudies-arrayexpress

Tissue-specific chromatin binding patterns of C. elegans heterochromatin proteins HPL-1 and HPL-2 reveal differential roles in the regulation of gene expression.

Project description:Tissue-specific chromatin binding patterns of C. elegans heterochromatin proteins HPL-1 and HPL-2 reveal differential roles in the regulation of gene expression.

2023-01-09 | GSE222056 | GEO

HPL-2 in N2 (wild-type) early embryo

Project description:ChIP-chip of HPL-2 in N2 C. elegans early embryo EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: N2; Developmental Stage: Early Embryo; Genotype: wild type; Sex: population predominantly Hermaphrodites perhaps with some Males; NUMBER OF REPLICATES: 3; EXPERIMENTAL FACTORS: temperature 20

2014-10-11 | E-GEOD-58762 | biostudies-arrayexpress

Transcriptome analysis of hpl-2(tm1489) vs wild-type worms at mixed embryonic and third larval stages

Project description:Epigenetic factors guide cell fate decisions and can stabilize development by buffering environmental variation. HP1 proteins have a well-characterized role in heterochromatin packaging and gene regulation, while their function in organismal development is less well understood. Here we used genome-wide expression profiling to assess novel functions of the C. elegans HP1 homologue HPL-2 at specific developmental stages RNA was extracted from either mixed-stage embryos or from third larval stage worms for both wild-type N2 (Bristol) and hpl-2(tm1489) strains.

2011-11-30 | E-GEOD-33700 | biostudies-arrayexpress

Identification of Transcription Factor HPL-2::GFP Binding Regions in L1

Project description:modENCODE_submission_3604 This submission comes from a modENCODE project of Michael Snyder. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Strain: YL416(official name : YL416 genotype : unc-119(ed3) III; vrIs64[pGES-1::HPL-2::GFP FLAG: HPL-2 3'-UTR genotype : unc-119 (+)] outcross : 0 mutagen : None tags : GFP::3xFlag description : The HPL-2::GFP fusion protein is driven by the ges-1 promoter and is expressed in the intestine. made_by : Michelle Kudron in Reinke lab ); Developmental Stage: fed L1; Genotype: unc-119(ed3) III; vrIs64[pGES-1::HPL-2::GFP FLAG: HPL-2 3'-UTR; Sex: Hermaphrodite; EXPERIMENTAL FACTORS: Developmental Stage fed L1; Target gene hpl-2; Strain YL416(official name : YL416 genotype : unc-119(ed3) III; vrIs64[pGES-1::HPL-2::GFP FLAG: HPL-2 3'-UTR genotype : unc-119 (+)] outcross : 0 mutagen : None tags : GFP::3xFlag description : The HPL-2::GFP fusion protein is driven by the ges-1 promoter and is expressed in the intestine. made_by : Michelle Kudron in Reinke lab ); temp (temperature) 20 degree celsius

2013-02-04 | E-GEOD-44017 | biostudies-arrayexpress

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