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Expression profiling of DT40 chicken B cell line by RNA-seq

ABSTRACT: A single replicate of exponentially growing DT40 CL18 chicken B lymphoma cells were harvested and extracted RNA was subjected to Illumina GAIIx paired-end sequencing to determine global gene expression.

ORGANISM(S): Gallus gallus

PROVIDER: GSE58766 | GEO | 2015/09/04

SECONDARY ACCESSION(S): PRJNA253403

REPOSITORIES: GEO

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Expression profiling of DT40 chicken B cell line by RNA-seq

Project description:A single replicate of exponentially growing DT40 CL18 chicken B lymphoma cells were harvested and extracted RNA was subjected to Illumina GAIIx paired-end sequencing to determine global gene expression. Single replicate RNA-seq expression analysis of DT40 cells.

2015-09-04 | E-GEOD-58766 | biostudies-arrayexpress

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Evaluation of the chicken transcriptome by SAGE of B cell and the DT40 cell line

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Project description:There are six histone H1 variant in chicken. 01H1/02H1/03H1/.10H1/H1L and H1R. In those variant we forcused on H1L and H1R those molecule were most similar H1 among six H1 variants. we established linker histone H1L and H1R deleted mutant in chicken B-cell derived DT40 cell and assay gene expression in normal condition in those mutant cells. The detail charactalization of those mutant cell was published in Takami et al., BBRC 268, 501-508 (2000) and Hashimoto et al., DNA repair (in press) Experiment Overall Design: There are six linker histone H1 variant in chicken. Linker histone was thought to be general gene supplesser. We established linker histone H1 variant mutant in chicken B-cell derived DT40 cell, and analyze total protein by 2D-PAGE to find up- or down- regulatd protein. In these mutant, there are some up regulated protein expression and down regulated protein expression (Takami et al., BBRC 268, 501-508 (2000), PubMedID 10679234). Experiment Overall Design: These 2D-PAGE experiment were limited at low density protein region, we use Affymetrix array to search the gene that were regulated specific linker histone H1 variant mutant. Experiment Overall Design: We assayed total gene expression profile in H1L and H1R deleted mutant cells. Experiment Overall Design: all mutant cells were cultured in normal culture condition in RPMI 1640 medium supplemented with 10 ÂµM 2-mercaptoethanol, 10% FCS (Biowest) and 1% chicken serum (Gibco) at 39.5ËC. Total RNA were isolated from exponently growing DT40 cells.

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DT40 genome

Project description:The genome of the DT40 chicken bursal lymphoma cell line

| PRJEB6887 | ENA

Expression data from chicken B cell lymphoma (DT40) cell lines.

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Transcription profiling of chicken B cell lymphoma (DT40) cell lines.

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