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RepliSeq of HT-1080 cell line

ABSTRACT: Analysis of replication timing by RepliSeq of HT-1080 Human Connective Tissue Fibrosarcoma Cells. HT-1080 subclone +4 cell line as described in “Human gene targeting favors insertions over deletions.” Russell, D.W., and Hirata, R.K. (2008). Hum Gene Ther 19, 907-914.

ORGANISM(S): Homo sapiens

PROVIDER: GSE58907 | GEO | 2014/07/31

SECONDARY ACCESSION(S): PRJNA253850

REPOSITORIES: GEO

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RepliSeq of HT-1080 cell line

Project description:Analysis of replication timing by RepliSeq of HT-1080 Human Connective Tissue Fibrosarcoma Cells. HT-1080 subclone +4 cell line as described in “Human gene targeting favors insertions over deletions.” Russell, D.W., and Hirata, R.K. (2008). Hum Gene Ther 19, 907-914. RepliSeq of HT-1080 cell line was used to determine the impact of DNA replication on targeted adeno-associated virus sites by mapping replication forks, which revealed a consistent preference for recombination at target loci transcribed towards an incoming fork.

2014-07-31 | E-GEOD-58907 | biostudies-arrayexpress

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Development of gene expression signatures for subconfluent and overconfluent HT-1080 fibrosarcoma cell line

Project description:We investigated the changes in gene expression profile between subconfluent (80%) and overconfluent HT-1080 fibrosarcoma cell line. We have employed whole genome microarray expression profiling as a discovery platform to identify genes expression profiles in HT-1080 cells. Total RNAs from subconfluent (HT-1080_80%) and overconfluent (HT-1080_overconfluent) HT-1080 cells were harvested and subjected to the microarray analysis.

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Development of gene expression signatures for subconfluent and overconfluent HT-1080 fibrosarcoma cell line

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Brevundimonas sp. 1080 isolate:DPS 1080

Project description:Brevundimonas sp. 1080 isolate:DPS 1080 Genome sequencing

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Genes regulated in HT-1080 cells after 24 hours of treatment with IRAK4 inhibitor

Project description:Analysis of HT-1080 fibrosarcoma cells transcriptome following 24 hours treatment with IRAK4 inhibitor was performed. Results provide insight into mode of action of IRAK4 inhibitor under study. Project co-financed from European Regional Development Fund under The Operational Program Innovative Economy, 2007-2013, measure 1.4 (UDA-POIG.01.04.00-12-049/11-00)

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Genes regulated in HT-1080 cells after 4 hours of treatment with IRAK4 inhibitor

Project description:Analysis of HT-1080 fibrosarcoma cells transcriptome following 4 hours treatment with IRAK4 inhibitor was performed. Results provide insight into mode of action of IRAK4 inhibitor under study. Project co-financed from European Regional Development Fund under The Operational Program Innovative Economy, 2007-2013, measure 1.4 (UDA-POIG.01.04.00-12-049/11-00)

2018-11-24 | GSE122844 | GEO

Aqueous and DMSO extracts from Annona muricata leaves protect from ferroptosis to both HT-1080 fibrosarcoma cells and HMEC-1 endothelial cells.

Project description:Graviola (Annona muricata) is a tropical plant with many traditional ethnobotanic uses and pharmacologic applications. A metabolomic study of both aqueous and DMSO extracts from Annona muricata leaves recently allowed us to identify dozens of bioactive compounds. In the present study, we use a proteomic study to reveal new bioactivities of these leave extracts on both conditioned media and extracts of HT-1080 fibrosarcoma treated cells. Our results reveal the complete sets of deregulated proteins after treatment with aqueous and DMSO extracts from An-nona muricata leaves. Functional enrichment analysis of proteomic data suggests deregulation of cell cycle and iron metabolism, which are experimentally validated. Additional experimental data reveal that these extracts protect from ferroptosis to both HT-1080 fibrosarcoma cells and HMEC-1 endothelial cells.

2023-10-24 | PXD042354 | Pride

Genes regulated in HT-1080 cells after treatment with IRAK4 inhibitor

Project description:Genes regulated in HT-1080 cells after treatment with IRAK4 inhibitor

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Dual-Isotope Lipidomics HT-1080 Ferroptosis

Project description:Full MS dataset from POS and NEG modes from lipidomic extraction of HT-1080 cells. C is control, D is treated with deuterated arachidonic acid (dAA), R is treated with RSL3, and RD is treated with both dAA and RSL3. This dataset accompanies a manuscript titled "Tracking the Fate of Exogenous Arachidonic Acid in Ferroptosis Using Dual-Isotope Labeling Lipidomics" (JASMS, 2023)

2023-06-27 | MSV000092288 | MassIVE

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