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Temporal expression profile of granulocytic differentiation of primary CD34+ cells cultured under 5% and 20% O2 levels

ABSTRACT: Little is known about the global transcriptional program underlying granulocytic (G) commitment, differentiation and maturation. Using DNA microarrays and Q-RT-PCR, we examined the transcriptional profile of G differentiation of human CD34-positive hematopoietic stem and progenitor cells cultured with interleukin-3, interleukin-6 granulocyte colony stimulating factor and stem cell factor. The goal this study was to identify genes involved in the various facets of G differentiation including commitment, expansion, differentiation and functional capacity. This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE5922 | GEO | 2007/06/06

SECONDARY ACCESSION(S): PRJNA97367

REPOSITORIES: GEO

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Temporal expression profile of granulocytic differentiation of primary CD34+ cells cultured under 20% O2 levels

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Project description:Little is known about the global transcriptional program underlying granulocytic (G) commitment, differentiation and maturation. Using DNA microarrays and Q-RT-PCR, we examined the transcriptional profile of G differentiation of human CD34-positive hematopoietic stem and progenitor cells cultured with interleukin-3, interleukin-6 granulocyte colony stimulating factor and stem cell factor. The goal this study was to identify genes involved in the various facets of G differentiation including commitment, expansion, differentiation and functional capacity. This SuperSeries is composed of the following subset Series:; GSE5917: Temporal expression profile of granulocytic differentiation of primary CD34+ cells cultured under 20% O2 levels; GSE5918: Temporal expression profile of granulocytic differentiation of primary CD34+ cells cultured under 5% O2 levels; GSE6792: The transcriptional patterns of mature normal PB neutrophils (granulocytes) was examined and used as a control against which the transcriptional programs of cultured granulocytes and granulocytic cells lines can be compared. Experiment Overall Design: Refer to individual Series

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2008-06-14 | E-GEOD-5917 | biostudies-arrayexpress

Temporal expression profile of granulocytic differentiation of primary CD34+ cells cultured under 5% O2 levels

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Granulocytic nuclear lobation.

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