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The Brucella abortus virulence regulator, LovhK, is a sensor kinase in the general stress response signaling pathway

ABSTRACT: We sequenced the transcriptomes of B. abortus 2308 wild type, rpoE1, and lovhK deletion strains to assess gene expression in response to oxidative stress. The data represent 2 independent biological replicates of each strain.

ORGANISM(S): Brucella melitensis

PROVIDER: GSE59513 | GEO | 2014/09/25

SECONDARY ACCESSION(S): PRJNA255490

REPOSITORIES: GEO

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The Brucella abortus virulence regulator, LovhK, is a sensor kinase in the general stress response signaling pathway

Project description:We sequenced the transcriptomes of B. abortus 2308 wild type, rpoE1, and lovhK deletion strains to assess gene expression in response to oxidative stress. The data represent 2 independent biological replicates of each strain. Illumina RNA-seq of total mRNA isolated from wild type, rpoE1, and lovhK deletion strains that were each exposed to 5 mM hydrogen peroxide for 10 min.

2014-09-25 | E-GEOD-59513 | biostudies-arrayexpress

Expression Data from Brucella abortus strain 2308 and isogenic mavR mutant cells

Project description:MavR is a 160 nucleotide regulatory RNA molecule that is produced during B. abortus strain 2308 growth in nutrient-replete broth. We used custom-made Affymetrix B. abortus strain 2308 derived GeneChips to compare the gene expression properties of wild type and isogenic mavR mutant cells.

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The endoribonuclease YbeY is linked to proper cellular morphology and virulence in Brucella abortus

Project description:The ybeY locus is hypothesized to produce an endoribonuclease, that is expressed during late exponential phase growth and regulate transcript titers. We used custom-made Affymetrix B. abortus strain 2308 derived GeneChips to compare the gene expression properties of wild type and isogenic ybeY mutant cells.

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Expression Data from Brucella abortus strain 2308 and isogenic vtlR mutant cells

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Project description:VtlR (virulence-associated transcriptional LysR-family regulator) is thought to be a transcriptional regulator of B. abortus virulence determinants We used custom-made Affymetrix B. abortus strain 2308 derived GeneChips to compare the gene expression properties of wild type and isogenic vtlR mutant cells. B. abortus strain 2308 or vltR mutant cells were grown to late exponential phase growth, total bacterial RNA was isolated and subjected to GeneChip hybridization and analysis. We sought to determine the regulatory effects of VtlR.

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The Brucella abortus general stress response system is required for chronic animal infection and exhibits noncanonical post-translational regulation

Project description:rpoE1 encodes an extracytoplasmic function (ECF) sigma factor to initiate the transcription of the genes responsible for general stress responses. This experiment is quantifying transcription in two Brucella strains: B. abortus 2308 wild type and rpoE1 in-frame deletion strains (gene bab1_1672).

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Expression Data from Brucella abortus strain 2308 and isogenic mucR mutant cells

Project description:MucR is one of the few transcriptional regulatory proteins that has been linked to Brucella pathogenesis. We used custom-made Affymetrix B. abortus strain 2308 derived GeneChips to copare the gene expression properties of wild type and isogenic mucR mutant cells. B. abortus strain 2308 or mucR mutant cells were grown to stationary phase growth, total bacterial RNA was isolated and subjected to GeneChip hybridization and analysis. We sought to determine the regulatory effects of MucR.

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Transcription profiling of B. abortus BvrS BvrR from B. abortus 2308

Project description:The analysis of the expression profile of the two component system BvrR/BvrS of the B. abortus 2308 making the comparison of the expression of the B. abortus 2308 and B. abortus 2308 BvrR- (mutant in the gen BvrR) whit the microarray of brucella melitensis (Brucearray)

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The Brucella abortus virulence regulator, LovhK, is a sensor kinase in the general stress response signalling pathway.

Project description:In the intracellular pathogen Brucella abortus, the general stress response (GSR) signalling system determines survival under acute stress conditions in vitro, and is required for long-term residence in a mammalian host. To date, the identity of the Brucella sensor kinase(s) that function to perceive stress and directly activate GSR signalling have remained undefined. We demonstrate that the flavin-binding sensor histidine kinase, LovhK (bab2_0652), functions as a primary B. abortus?GSR sensor. LovhK rapidly and specifically phosphorylates the central GSR regulator, PhyR, and activates transcription of a set of genes that closely overlaps the known B. abortus?GSR regulon. Deletion of lovhK severely compromises cell survival under defined oxidative and acid stress conditions. We further show that lovhK is required for cell survival during the early phase of mammalian cell infection and for establishment of long-term residence in a mouse infection model. Finally, we present evidence that particular regions of primary structure within the two N-terminal PAS domains of LovhK have distinct sensory roles under specific environmental conditions. This study elucidates new molecular components of a conserved signalling pathway that regulates B. abortus stress physiology and infection biology.

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Expression Data from Brucella abortus strain 2308 and isogenic mucR mutant cells

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