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Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress [set1]

ABSTRACT: We use RNA-Seq to investigate gene expression differences in response to saltwater exposure by the larval stages of the obligate freshwater mosquito Anopheles coluzzii (formerly An. gambiae "M" form), and the euryhaline An. merus.

ORGANISM(S): Anopheles coluzzii Anopheles merus

PROVIDER: GSE59632 | GEO | 2016/03/09

SECONDARY ACCESSION(S): PRJNA255780

REPOSITORIES: GEO

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Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress [set1]

Project description:We use RNA-Seq to investigate gene expression differences in response to saltwater exposure by the larval stages of the obligate freshwater mosquito Anopheles coluzzii (formerly An. gambiae "M" form), and the euryhaline An. merus. After rearing in freshwater, both young and old larval instars of each species were briefly (6 h) exposed to either saltwater (SW) or freshwater (FW) conditions to test the impact of salinity on mRNA levels.

2016-03-09 | E-GEOD-59632 | biostudies-arrayexpress

Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress [set2]

Project description:We use RNA-Seq to explore the reaction norm of gene expression of larval Anopheles coluzzii and An. merus to a gradient of salinity.

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Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress [set2]

Project description:We use RNA-Seq to explore the reaction norm of gene expression of larval Anopheles coluzzii and An. merus to a gradient of salinity. After rearing in freshwater, separate pools of 18 h post-hatch larval instars of An. merus and An. coluzzii were briefly (6 h) exposed to one of six water salinities: 0%, 10%, 20%, 30%, 40%, or 50% the salinity of seawater. The last sample (G-50-RC) was split across 5 lanes for sequencing, resulting in total 10 fastq files associated with subparts G-50-RCa, G-50-RCb, G-50-RCc, G-50-RCd, G-50-RCe.

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Project description:Transcription profiles of three field collections of Anopheles gambiae M molecular form (i.e. Anopheles coluzzii) from West Africa were compared to investigate their phenotypic differences in insecticide resistance. The susceptibility status for the individual samples was unknown but known for their source populations via phenotypic assays performed concurrently with the sample collections.

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Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress [set1]

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