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Toxicity Evaluation of Manufactured CeO2 Nanoparticles Before and After Alteration: Combined Physicochemical and Whole-Genome Expression Analysis in Caco-2 Cells.

ABSTRACT: We have employed whole genome microarray expression to distinguish the effect of environmental aging on the toxicity of several cerium oxide nanoparticles (NPs) in human intestinal cells compared . Cells were exposed in vitro, and datasets of differentially expressed genes were identified for each type of NPs versus control samples.

ORGANISM(S): Homo sapiens

PROVIDER: GSE60128 | GEO | 2014/10/01

SECONDARY ACCESSION(S): PRJNA257671

REPOSITORIES: GEO

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Toxicity Evaluation of Manufactured CeO2 Nanoparticles Before and After Alteration: Combined Physicochemical and Whole-Genome Expression Analysis in Caco-2 Cells.

Project description:We have employed whole genome microarray expression to distinguish the effect of environmental aging on the toxicity of several cerium oxide nanoparticles (NPs) in human intestinal cells compared . Cells were exposed in vitro, and datasets of differentially expressed genes were identified for each type of NPs versus control samples. NPs induced gene expression in Caco-2 cells was measured at 24 hours after exposure . Six independent experiments were performed using different NPs and controls for each experiment.

2014-10-01 | E-GEOD-60128 | biostudies-arrayexpress

RNAseq of human epithelial lung carcinoma cell line A549 exposed to ceria, silver or zinc ions, micron-sized particles or nanoparticles

Project description:The toxicity of silver and zinc oxide nanoparticles is hypothesised to be mediated by dissolved metal ions and cerium dioxide nanoparticles (CeO2 NPs) are hypothesised to induce toxicity specifically by oxidative stress dependant on their surface redox state. To test these hypotheses, RNAseq was applied to characterise the molecular responses of cells to metal nanoparticle and metal ion exposures. The human epithelial lung carcinoma cell line A549 was exposed to different CeO2 NPs with different surface charges, micron-sized and nano-sized silver particles and silver ions, micron-sized and nano-sized zinc oxide particles and zinc ions, or control conditions, for 1 hour, 6 hours and 24 hours. Concentrations were the lower of either EC20 or 128 micrograms/mL. Transcriptional responses were characterised by RNAseq transcriptomics using an Illumina HiSeq2500 .

2018-02-01 | E-MTAB-5734 | biostudies-arrayexpress

Gene expression profiles under oxidative stress conditions in human HaCaT keratinocytes and the derivative cells expressing shRNA against NRF2, MLL1, or UTX.

Project description:To determine how transcriptome is altered by knockdown of NRF2, MLL1, or UTX, we performed RNA-sequencing (RNA-seq) analysis of HaCaT cells and its shRNA-expressing derivatives before and after exposure to hydrogen peroxide (H2O2) or cerium oxide nanoparticles (CeO2 NPs).

2024-03-27 | GSE250127 | GEO

Gene expression profile in zebrafish D. rerio exposed to metal oxidate nanoparticles

Project description:Metal oxide nanoparticles can exert adverse effects on humans and aquatic organisms. However, the toxic effects and mechanisms of MO-NPs are not clearly understood.We investigated the toxic effects and mechanisms of copper oxide, zinc oxide, and nickel oxide nanoparticles in Danio rerio using microarray analysis.

2018-03-20 | GSE112016 | GEO

Project description:This study investigated the pulmonary toxicity of Copper oxide nanoparticles (CuO NPs) surface modified with polyethylenimine (PEI) or ascorbate (ASC), was investigated. Rats were exposed nose-only to a fixed exposure concentration of ASC or PEI coated CuO NPs for 5 consecutive days. On day 6 and day 27 post-exposure, pulmonary toxicity markers in bronchoalveolar lavage fluid (BALF) were analyzed and histopathological evaluation of the lungs was performed, along with microarray analyses on whole lung tissue samples.

2020-10-02 | GSE158900 | GEO

Cerium_Oxide_Nanoparticles_e_coli

Project description:The effects of cerium oxide nanoparticles on the gene expression of Gram-negative Escherichia coli is tested. 8 cultures of E. coli were treated with either cerium nanoparticles or cerium chloride solution at 50 mgs/ml. 4 cultures were untreated.

2010-08-20 | E-GEOD-20326 | biostudies-arrayexpress

RNA-seq of C17.2 neuronal progenitor cells exposed to cerium oxide nanoparticles during cell differentiation

Project description:We used a next-generation sequencing approach to understand the effects of antioxidant cerium oxide nanoparticles (CeO2) on neuronal stem cell differentiation. As a model we used the murine neuronal progenitor cell line, C17.2, which upon differentiation, is able to generate a mixed culture of neurons and neuroglial cells. As additional controls we used N-acetylcysteine (NAC), a conventional antioxidant and samarium doped cerium oxide nanoparticles (Sm-CeO2), as particle controls (as they bear a reduced antioxidant potential as compared to CeO2 alone). We had a time series approach and we investigates effects after 1, 4 and 7 days during differentiation. We revealed that CeO2 reduce axonal guidance signalling, neuronal differentiation and neuroglial differentiation after 7 days, thus having a negative effect on neuronal development. Overall, these effects are likely due to the antioxidant properties of nanoceria, although some evidence for a particle effect was also provided as indicated by the interference with cytoskeletal as well as integrin signaling genes both by nanoceria and Sm-doped nanoceria, but not by NAC.

2017-08-25 | E-MTAB-4398 | biostudies-arrayexpress

Toxicity and transcriptomic analysis in Hyalella azteca suggests increased exposure and susceptibility of epibenthic organisms to Zinc Oxide Nanoparticles

Project description:Abstract: Nanoparticles (NPs) are expected to make their way into the aquatic environment where sedimentation of particles will likely occur, putting benthic organisms at particular risk. Therefore, organisms such as Hyalella azteca, an epibenthic crustacean which forages at the sediment surface, is likely to have a high potential exposure. Here we show that Zinc Oxide (ZnO) NPs are more toxic to H. azteca compared with the corresponding metal ion, Zn2+. Dissolution of ZnO NPs contributes about 50% of the Zn measured in the ZnO NP suspensions, and cannot account for the toxicity of these particles to H. azteca. However, gene expression analysis is unable to distinguish between the ZnO NP exposures and Zinc Sulfate (ZnSO4) exposures at equitoxic concentrations. These results lead us to hypothesize that ZnO NPs provide and an enhanced exposure route for Zn2+ uptake into H. azteca, and possibly other sediment dwelling organisms. Our study supports the prediction that sediment dwelling organisms are highly susceptible to the effects of ZnO NPs and should be considered in the risk assessment of these nanomaterials. This experiment included four different treatments and an untreated control. Each treatment or control, consisted of ten independent replicates of twenty Hyalella azteca. Of these, six were randomly chosen to be used for the microarray analysis.

2013-07-24 | E-GEOD-49136 | biostudies-arrayexpress

Cerium_Oxide_Nanoparticles_e_coli

Project description:The effects of cerium oxide nanoparticles on the gene expression of Gram-negative Escherichia coli is tested.

2010-08-20 | GSE20326 | GEO

Molecular responses of human lung epithelial cells to the toxicity of copper oxide nanoparticles inferred from whole genome expression analysis.

Project description:This study proposes a molecular mechanism for lung epithelial A549 cell response to copper oxide nanoparticles (CuO-NPs) related to Cu ions released from CuO-NPs. Cells that survived exposure to CuO-NPs arrested the cell cycle as a result of the downregulation of proliferating cell nuclear antigen (PCNA), cell division control 2 (CDC2), cyclin B1 (CCNB1), target protein for Xklp2 (TPX2), and aurora kinase A (AURKA) and B (AURKB). Furthermore, cell death was avoided through the induced expression of nuclear receptors NR4A1 and NR4A3 and growth arrest and DNA damage-inducible 45 Î² and Î³ (GADD45B and GADD45G, respectively). The downregulation of CDC2, CCNB1, TPX2, AURKA, and AURKB, the expressions of which are involved in cell cycle arrest, was attributed to Cu ions released from CuO-NPs into medium. NR4A1 and NR4A3 expression was also induced by Cu ions released into the medium. The expression of GADD45B and GADD45G activated the p38 pathway that was involved in escape from cell death. The upregulation of GADD45B and GADD45G was not observed with Cu ions released into medium but was observed in cells exposed to CuO-NPs. However, because the expression of the genes was also induced by Cu ion concentrations higher than that released from CuO-NPs into the medium, the expression appeared to be triggered by Cu ions released from CuO-NPs taken up into cells. We infer that, for cells exposed to CuO-NPs, those able to make such a molecular response survived and those unable to do so eventually died. Two-condition experiment, CuO-NPs exposured vs. non-treated cells. Hybridization: 2 replicates. Scanning: 3 replicates (Gain changed).

2012-01-05 | E-GEOD-33278 | biostudies-arrayexpress

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