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Gene expression profiles under oxidative stress conditions in human HaCaT keratinocytes and the derivative cells expressing shRNA against NRF2, MLL1, or UTX.

ABSTRACT: To determine how transcriptome is altered by knockdown of NRF2, MLL1, or UTX, we performed RNA-sequencing (RNA-seq) analysis of HaCaT cells and its shRNA-expressing derivatives before and after exposure to hydrogen peroxide (H2O2) or cerium oxide nanoparticles (CeO2 NPs).

ORGANISM(S): Homo sapiens

PROVIDER: GSE250127 | GEO | 2024/03/27

REPOSITORIES: GEO

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Genomic occupancies of MLL1 and UTX, and H3K4me3/K3K27me3 histone modifications upon hydrogen peroxide treatment in human HaCaT keratinocytes.

Project description:ChIP-seq analysis was conducted to investigate the effects of hydrogen peroxide (H2O2) treatment on the occupancy of MLL1 and UTX, and the associated histone modifications in HaCaT cells.

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Gene expression profiles under oxidative stress conditions in human HaCaT keratinocytes and the derivative cells expressing shRNA against NRF2, MLL1, or UTX.

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Genomic occupancies of MLL1 and UTX, and H3K4me3/K3K27me3 histone modifications upon hydrogen peroxide treatment in human HaCaT keratinocytes.

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Project description:Immunoprecipitation of Utx followed by mass spectrometry analysis to identify Utx binding partners in mouse myeloid progenitor cells.

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RNA-seq of C17.2 neuronal progenitor cells exposed to cerium oxide nanoparticles during cell differentiation

Project description:We used a next-generation sequencing approach to understand the effects of antioxidant cerium oxide nanoparticles (CeO2) on neuronal stem cell differentiation. As a model we used the murine neuronal progenitor cell line, C17.2, which upon differentiation, is able to generate a mixed culture of neurons and neuroglial cells. As additional controls we used N-acetylcysteine (NAC), a conventional antioxidant and samarium doped cerium oxide nanoparticles (Sm-CeO2), as particle controls (as they bear a reduced antioxidant potential as compared to CeO2 alone). We had a time series approach and we investigates effects after 1, 4 and 7 days during differentiation. We revealed that CeO2 reduce axonal guidance signalling, neuronal differentiation and neuroglial differentiation after 7 days, thus having a negative effect on neuronal development. Overall, these effects are likely due to the antioxidant properties of nanoceria, although some evidence for a particle effect was also provided as indicated by the interference with cytoskeletal as well as integrin signaling genes both by nanoceria and Sm-doped nanoceria, but not by NAC.

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