A streamline experimental and computational approach for inexpensive, accurate, efficient and high-throughput annotation of 5’ and 3’ ends

ABSTRACT: We present a simple and straightforward approach for genome-wide annotation of 5' and 3'-RNA ends. Our approach reliably discerns bona-fide PAS from false PAS that arise due to internal poly(A) tracts, a common problem with current PAS annotation methods. We applied our methodology to study the impact of temperature on the Drosophila melanogaster head transcriptome. We found hundreds of previously unidentified TSS and PAS which revealed two interesting phenomena: First, genes with multiple PASs tend to harbor a motif near the most proximal PAS, which likely represents a new cleavage and polyadenylation signal. Second, motif analysis of promoters of genes affected by temperature suggested that BEAF-32 and DREF mediates a transcriptional program at warm temperatures, a result we validated in a fly line where beaf-32 is downregulated.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE60215 | GEO | 2017/02/13

SECONDARY ACCESSION(S): PRJNA257775

REPOSITORIES: GEO