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AUTS2 confers transcriptional activation to PRC1 in the CNS

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE60411 | GEO | 2014/12/18

SECONDARY ACCESSION(S): PRJNA258198

REPOSITORIES: GEO

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AUTS2 confers transcriptional activation to PRC1 in the CNS

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-12-18 | E-GEOD-60411 | biostudies-arrayexpress

AUTS2 confers transcriptional activation to PRC1 in the CNS (ChIP-Seq)

Project description:Naturally occurring variations of Polycomb Repressive Complex 1 (PRC1) comprise a core assembly of Polycomb group proteins and additional factors that include, surprisingly, Autism Susceptibility Candidate 2 (AUTS2). While AUTS2 is often disrupted in patients with neuronal disorders, the underlying mechanism is unclear. We investigated the role of AUTS2 as part of a previously identified PRC1 complex (PRC1-AUTS2), and in the context of neurodevelopment. In contrast to the canonical role of PRC1 in gene repression, PRC1-AUTS2 activates transcription. Biochemical studies demonstrate that the CK2 component of PRC1-AUTS2 thwarts PRC1 repressive activity and AUTS2-mediated recruitment of P300 leads to gene activation. ChIP-seq of AUTS2 shows that it regulates neuronal gene expression through promoter association. Conditional CNS targeting of Auts2 in a mouse model leads to various developmental defects. These findings reveal a natural means of subverting PRC1 activity, linking key epigenetic modulators with neuronal functions and diseases. ChIP-seq experiments of PRC1 components, PolII as well as histone modifications were performed either in mouse whole brain or in human 293T-REx lines expressing FLAG/HA-tagged subunits. RNA-seq was performed to analyze the expression profile of mouse whole brain.

2014-12-18 | E-GEOD-60409 | biostudies-arrayexpress

AUTS2 confers transcriptional activation to PRC1 in the CNS (RNA-Seq)

Project description:Naturally occurring variations of Polycomb Repressive Complex 1 (PRC1) comprise a core assembly of Polycomb group proteins and additional factors that include, surprisingly, Autism Susceptibility Candidate 2 (AUTS2). While AUTS2 is often disrupted in patients with neuronal disorders, the underlying mechanism is unclear. We investigated the role of AUTS2 as part of a previously identified PRC1 complex (PRC1-AUTS2), and in the context of neurodevelopment. In contrast to the canonical role of PRC1 in gene repression, PRC1-AUTS2 activates transcription. Biochemical studies demonstrate that the CK2 component of PRC1-AUTS2 thwarts PRC1 repressive activity and AUTS2-mediated recruitment of P300 leads to gene activation. ChIP-seq of AUTS2 shows that it regulates neuronal gene expression through promoter association. Conditional CNS targeting of Auts2 in a mouse model leads to various developmental defects. These findings reveal a natural means of subverting PRC1 activity, linking key epigenetic modulators with neuronal functions and diseases. mRNA profiles of P1 brain from wild type mice were generated by deep sequencing

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AUTS2 confers transcriptional activation to PRC1 in the CNS (ChIP-Seq)

2014-12-18 | GSE60409 | GEO

AUTS2 confers transcriptional activation to PRC1 in the CNS (RNA-Seq)

2014-12-18 | GSE60410 | GEO

AUTS2 confers transcriptional activation to PRC1 in the CNS (ChIP-Seq)

Project description:AUTS2 confers transcriptional activation to PRC1 in the CNS (ChIP-Seq)

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Project description:This SuperSeries is composed of the SubSeries listed below.

2012-07-12 | GSE37657 | GEO

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