Transcriptomics

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RNA-seq characterization of an infection of Mycobacterium smegmatis by a cluster A4 mycobacteriophage


ABSTRACT: We present transcriptomic analysis of an infection by a cluster A4 mycobacteriophage of its host, Mycobacterium smegmatis, at six time points (n = 2). Viral transcription is detectable 5 minutes into infection, and viral mRNA makes up the vast majority of all transcription by 30 minutes. Kampy, like many phages, has a genome organized into two arms bearing early- and late-phase genes. We identify two transcriptional start sites (TSS’s) consistent with an early promoter driving expression of half of the genome, along with a second, late-stage promoter that becomes active around 30 minutes after the start of infection.

ORGANISM(S): Mycolicibacterium smegmatis Mycobacterium phage Kampy

PROVIDER: GSE60592 | GEO | 2015/02/02

SECONDARY ACCESSION(S): PRJNA258602

REPOSITORIES: GEO

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