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Early life Lead exposure causes distinct gender specific changes in the DNA methylation profile of DNA extracted from dried blood spots

ABSTRACT: Study of influence of gender and Lead(Pb) exposure on DNA methylation for whole blood measured in dried blood spots for a Detroit cohort of child between the age of 3 months to 5years

ORGANISM(S): Homo sapiens

PROVIDER: GSE60598 | GEO | 2014/09/01

SECONDARY ACCESSION(S): PRJNA258633

REPOSITORIES: GEO

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Early life Lead exposure causes distinct gender specific changes in the DNA methylation profile of DNA extracted from dried blood spots

Project description:Study of influence of gender and Lead(Pb) exposure on DNA methylation for whole blood measured in dried blood spots for a Detroit cohort of child between the age of 3 months to 5years DNA methylation profiling of dried blood spots for a Detroit cohort of child between the ages of 3 months to 5 years Differential methylation study

2014-09-01 | E-GEOD-60598 | biostudies-arrayexpress

Early life lead exposure causes gender-specific changes in the DNA methylation profile of DNA extracted from dried blood spots.

Project description:AimsIn this paper, we tested the hypothesis that early life lead (Pb) exposure associated DNA methylation (5 mC) changes are dependent on the sex of the child and can serve as biomarkers for Pb exposure.MethodsIn this pilot study, we measured the 5mC profiles of DNA extracted from dried blood spots (DBS) in a cohort of 43 children (25 males and 18 females; ages from 3 months to 5 years) from Detroit. Result & Discussion: We found that the effect of Pb-exposure on the 5-mC profiles can be separated into three subtypes: affected methylation loci which are conserved irrespective of the sex of the child (conserved); affected methylation loci unique to males (male-specific); and affected methylation loci unique to females (female-specific).

| S-EPMC4501025 | biostudies-literature

Multigenerational epigenetic inheritance in humans: DNA methylation changes associated with maternal exposure to lead can be transmitted to the grandchildren

Project description:We report that the DNA methylation profile of a child’s neonatal whole blood can be significantly influenced by his or her mother’s neonatal blood lead levels (BLL). We recruited 35 mother-infant pairs in Detroit and measured the whole blood lead (Pb) levels and DNA methylation levels at over 450,000 loci from current blood and neonatal blood from both the mother and the child. We found that mothers with high neonatal BLL correlate with altered DNA methylation at 564 loci in their children’s neonatal blood. Our results suggest that Pb exposure during pregnancy affects the DNA methylation status of the fetal germ cells, which leads to altered DNA methylation in grandchildren’s neonatal dried blood spots. This is the first demonstration that an environmental exposure in pregnant mothers can have an epigenetic effect on the DNA methylation pattern in the grandchildren. For the study, we selected 35 dried blood spots (DBS) collected from mother-infant pairs from Health Fairs ran in three Detroit communities, because they have a high prevalence (8-11%) of high BLL in children. The sample set consisted of 25 male children and 18 female children. We also collected the neonatal DBS and mother neonatal DBS for these mother-infant pairs from the Michigan Neonatal Biobank. Then we measured a blood lead levels in dried blood spots using using atomic absorbtion spectrophotometry. Finally we measured the DNA methylation levels using human methylation 450K array from Illumina. Then we normalized the data for technical biases and tried to infer the the locus specific DNA methylation changes due to Pb exposure which was carried over from the grandmothers to the grandchildren by the Pb –exposed fetal germ cells of the mother using statistical model proposed by Sofer et al, 2012.

2015-04-01 | E-GEOD-67444 | biostudies-arrayexpress

Multigenerational epigenetic inheritance in humans: DNA methylation changes associated with maternal exposure to lead can be transmitted to the grandchildren

2015-04-01 | GSE67444 | GEO

Epigenome wide DNA methylation profile of neonatal blood spots from the Archives for Research on Child Health (ARCH) cohort

Project description:Genome wide DNA methylation profiling of human samples from dried neonatal blood spots taken at birth from a cohort of pregnant people and their infants in Michigan. The Illumina MethylationEPIC Beadchip array was used to obtain DNA methylation profiles across over 850,000 CpGs in dried neonatal blood spot samples. Samples were collected from 166 infants at birth.

2022-01-31 | GSE195595 | GEO

Mapping differences in salivary gland secretions using dried saliva spots

Project description:Dried saliva spot (DSS) sampling is a minimally invasive extraction technique that allows for the spatial mapping of salivary protein distribution in the oral cavity. In conjunction with untargeted nanoLC-Orbitrap-MS/MS analysis, we used DSS to compare the unstimulated proteomes secreted by the parotid and submandibular/sublingual salivary glands

2019-09-04 | PXD012792 | Pride

A nested real-time PCR assay for the quantification of Plasmodium falciparum DNA extracted from dried blood spots.

Project description:BackgroundAs public health efforts seek to eradicate malaria, there has been an emphasis on eliminating low-density parasite reservoirs in asymptomatic carriers. As such, diagnosing submicroscopic Plasmodium infections using PCR-based techniques has become important not only in clinical trials of malaria vaccines and therapeutics, but also in active malaria surveillance campaigns. However, PCR-based quantitative assays that rely on nucleic acid extracted from dried blood spots (DBS) have demonstrated lower sensitivity than assays that use cryopreserved whole blood as source material.MethodsThe density of Plasmodium falciparum asexual parasites was quantified using genomic DNA extracted from dried blood spots (DBS) and the sensitivity of two approaches was compared: quantitative real-time PCR (qPCR) targeting the P. falciparum 18S ribosomal RNA gene, either with an initial conventional PCR amplification prior to qPCR (nested qPCR), or without an initial amplification (qPCR only). Parasite densities determined by nested qPCR, qPCR only, and light microscopy were compared.ResultsNested qPCR results in 10-fold higher sensitivity (0.5 parasites/μl) when compared to qPCR only (five parasites/ul). Among microscopy-positive samples, parasite densities calculated by nested qPCR correlated strongly with microscopy for both asymptomatic (Pearson's r=0.58, P<0.001) and symptomatic (Pearson's r=0.70, P<0.0001) P. falciparum infections.ConclusionNested qPCR improves the sensitivity for the detection of P. falciparum blood-stage infection from clinical DBS samples. This approach may be useful for active malaria surveillance in areas where submicroscopic asymptomatic infections are prevalent.

| S-EPMC4197274 | biostudies-literature