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Expression data from mouse embryonic ureteric bud (UB) cells

ABSTRACT: Dicer-dependent miRNAs are required for UB morphogenesis and differentiation during metanephric kidney development. We used microarray analysis to identify genes whose expression in the UB epithelium are altered from UB-specific ablation of Dicer.

ORGANISM(S): Mus musculus

PROVIDER: GSE60758 | GEO | 2014/08/27

SECONDARY ACCESSION(S): PRJNA259441

REPOSITORIES: GEO

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Expression data from mouse embryonic ureteric bud (UB) cells

Project description:Dicer-dependent miRNAs are required for UB morphogenesis and differentiation during metanephric kidney development. We used microarray analysis to identify genes whose expression in the UB epithelium are altered from UB-specific ablation of Dicer. E15.5 UB cells from control and Dicer mutant kidneys were FACS sorted for transcription profiling.

2014-08-27 | E-GEOD-60758 | biostudies-arrayexpress

Hepatocyte nuclear factor 1β gene regulation in E14.5 ureteric bud cells

Project description:HNF-1β mutations are one of the most common single-gene mutations that underlie kidney developmental disease. Hepatocyte nuclear factor 1β (HNF-1β) is essential for kidney development, but its functions in ureteric bud (UB) branching morphogenesis are incompletely understood. We isolated E14.5 UB cells using fluorescence-activated cell sorting, and performed RNA-sequencing to compare gene expression in wild-type and HNF-1β deficient UB cells. 1632 genes have significantly lower expression in the HNF-1β deficient UB cells and 2223 genes have significantly higher expression in HNF-1β deficient UB cells.

2022-10-20 | GSE205217 | GEO

miRNA expression data from embryonic ureteric bud (UB) cells

Project description:UB cells FACS sorted from E15.5 mouse kidneys were analyzed for miRNAs expression. E15.5 UB cells FACS sorted from Rosa26YFP; Hoxb7Cre mice, total RNAs including miRNAs were purified and used for miRNA microarray

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Expansion of human iPSC derived ureteric bud organoids with repeated branching potential

Project description:We report a new method to generate UB organoids that possess epithelial polarity and tubular lumen and repeat branching morphogenesis. We also succeeded in monitoring UB tip cells by utilizing the ability of tip cells to uptake very-low-density lipoprotein, cryopreserving UB progenitor cells and expanding UB tip cells that can reconstitute the organoids and differentiate into collecting duct progenitors. Moreover, we successfully reproduced some phenotypes of multicystic dysplastic kidney (MCDK) using the UB organoids.

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miRNA expression data from embryonic ureteric bud (UB) cells

Project description:UB cells FACS sorted from E15.5 mouse kidneys were analyzed for miRNAs expression.

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Expansion of human iPSC-derived ureteric bud organoids with repeated branching potential [DiI-VLDL]

Project description:Ureteric bud (UB) is the embryonic kidney progenitor tissue that gives rise to the collecting duct and lower urinary tract. UB-like structures generated from human pluripotent stem cells by previously reported methods show limited developmental ability and limited branching. Here we report a new method to generate UB organoids that possess epithelial polarity and tubular lumen and repeat branching morphogenesis. We also succeeded in monitoring UB tip cells by utilizing the ability of tip cells to uptake very-low-density lipoprotein, cryopreserving UB progenitor cells and expanding UB tip cells that can reconstitute the organoids and differentiate into collecting duct progenitors. Moreover, we successfully reproduced some phenotypes of multicystic dysplastic kidney (MCDK) using the UB organoids. These methods will help elucidate the developmental mechanisms of UB branching and develop a selective differentiation method for collecting duct cells, contributing to the creation of disease models for congenital renal abnormalities.

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Expansion of human iPSC-derived ureteric bud organoids with repeated branching potential [CDP & TIP]

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Mouse embryonic kidneys (E13.5): mutant (UB HDAC1,2-/-) vs. wild type

Project description:Transcriptional profiling of mouse embryonic kidneys (E13.5) comparing UB HDAC1,2-/- kidneys with wild type kidneys. Studies in our lab showed that histone deacetylase 1 (HDAC1) and 2 (HDAC2) perform redundant, yet essential functions in the developing mouse ureteric bud (UB) tissue. Double deletion of HDAC1 and HDAC2 in the UB results in impaired UB branching morphogenesis, followed by severe kidney dysgenesis. The goal of the microarray analysis was to identify the genetic pathways controlled by HDAC1 and 2 in the UB.

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Mouse embryonic kidneys (E13.5): mutant (UB HDAC1,2-/-) vs. wild type

2012-01-31 | E-GEOD-35432 | biostudies-arrayexpress

Transcription profiling of wild type and HoxA11 metenephric mesenchyme and ureteric bud from embryonic day 11.5 mice

Project description:E11.5 metanephric mesenchyme and ureteric bud were dissected from the E11.5 kidney rudiment using fine manual microdissection (ureteric bud only) or both fine manual microdissection and laser capture microdissection (metanephric mesenchyme) to define the gene expression profiles of these structures. Additionally, HoxA11, HoxD11 compound null E11.5 metanephric mesenchyme was obtained through laser capture microdissection allowing analysis of possible Hox targets in kidney development. Targets from multiple biological replicates of each were generated and the expression profiles were determined using Affymetrix MOE430_v2 arrays. Using microdissection techniques, ureteric bud and metanephric mesenchyme were dissected from E11.5 kidney rudiments allowing the identificated genes specifically regulated in either structure. In addition, Hoxa11, Hoxd11 compound null E11.5 metanephric mesenchyme were normalized to wild type embryonic controls allowing the identification of potential Hox targets in normal kidney development. Each structure/genotype were represented in biological (seperate embryo) replicate.

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