Genomics

Dataset Information

0

Tumor circulating DNA profiling in xenografted mice exposed to intermittent hypoxia


ABSTRACT: Intermittent hypoxia (IH) is a hallmark of obstructive sleep apnea (OSA), which has been proposed as the major determinant of processes involving tumor invasion and metastasis. To study whether circulating DNA (cirDNA) in blood plasma reflects the changes that the tumor cells undergo under IH conditions, we used a xenografted murine model. Mice engrafted with TC1 epithelial lung and controls were exposed to IH or room air (RA) conditions. Plasma cirDNA amounts were significantly increased in mice exposed to IH (p<0.05). We found a significant correlation between plasma cirDNA concentration and tumor size, weight and invasiveness (p<0.05). Using a microarray-based approach, we identified 2,094 regions showing significant differential cirDNA modifications. System biology analysis revealed an association with molecular pathways misregulated in cancer progression and with distal and TSS-associated transcription factor binding sites. We detected clusters of highly variable regions in chromosomes 7, 13, 14 and X, which may highlight hotspots for DNA deletions. Single locus displayed high intragroup variation, suggesting cellular heterogeneity within the tissue may be associated to cirDNA release. Our result showed that exposure to IH increases the shedding of cirDNA into circulation, which carries epigenetic modifications that may characterize cell populations within the tumor that preferentially release their DNA upon IH exposure.

ORGANISM(S): Mus musculus

PROVIDER: GSE61070 | GEO | 2015/02/24

SECONDARY ACCESSION(S): PRJNA260179

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2015-02-24 | E-GEOD-61070 | biostudies-arrayexpress
2016-01-07 | E-GEOD-74145 | biostudies-arrayexpress
2016-01-07 | GSE74145 | GEO
2015-07-27 | GSE71356 | GEO
2010-12-31 | GSE21409 | GEO
2024-02-14 | GSE242668 | GEO
2023-12-28 | GSE235867 | GEO
2010-12-31 | E-GEOD-21409 | biostudies-arrayexpress
2021-05-12 | GSE162905 | GEO
2021-02-18 | GSE145434 | GEO