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Expression data from mammary epithelial cells harvested from 8-9 week old C3H female mice - various isolation conditions

ABSTRACT: Numerous lineage analysis studies have used FACS sorting as a separation technique prior to measuring mRNA expression patterns. To assess if FACS sorting causes non-specific changes in gene expression, we collected a heterogeneous population of mammary epithelial cells from three biological replicates. We then performed gene expression analysis on each replicate either prior to trypsin digestion, immediately following trypsin digestion, or following both the trypsin digestion and a mock FACS sort. In this dataset, we include gene expression data obtained from isolated murine mammary epithelial cells either prior to trypsin digestion, immediately following trypsin digestion, or following the trypsin digestion and a mock FACS sort.

ORGANISM(S): Mus musculus

PROVIDER: GSE61167 | GEO | 2014/09/05

SECONDARY ACCESSION(S): PRJNA260385

REPOSITORIES: GEO

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Expression data from mammary epithelial cells harvested from 8-9 week old C3H female mice - various isolation conditions

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