Delineating Tumor-infiltrating Antigen Presenting Cell populations

ABSTRACT: The goal of this study is to compare tumor-infiltrating antigen presenting cell populations by global transcriptome profiling (RNA-seq) to help further delineate sub-populations of infiltrating myeloid cells in tumor. Methods: Four tumor antigen presenting cell populations were sorted from digested B78chOVA (melanoma variant) tumors in biological triplicate Results: RNA was extracted from the 4 groups (n=3 per group) and prepared for RNAseq. Sequencing yielded ~405 million reads with an average read depth of 33.7 million reads/sample. Reads were then aligned to the mouse genome (UCSC mm10) and those that mapped uniquely to known mRNAs were used to assess differential expression.

ORGANISM(S): Mus musculus

PROVIDER: GSE61462 | GEO | 2014/11/10

SECONDARY ACCESSION(S): PRJNA261162

REPOSITORIES: GEO