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Escherichia coli MG1655 whole-genome tiling microarray design and validation

ABSTRACT: This research focuses on the design, manufacturing and validation of a new E. coli whole-genome tiling micraorray platform for novel RNA transcript discovery. A whole-genome tiling microarray allows both annotated genes as well as previously unknown RNA transcripts to be detected and quantified at once. The E. coli MG1655 genome is re-acquired with next-generation sequencing and then used to design the tiling microarray with the thermodynamic analysis program Picky. Validations are performed by subjecting E. coli under various growth conditions and then using the tiling microarrays to verify expected gene expression patterns.

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

PROVIDER: GSE61736 | GEO | 2014/09/25

SECONDARY ACCESSION(S): PRJNA261975

REPOSITORIES: GEO

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