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Host transcriptome analysis of Aspergillus fumigatus infection in Airway Epithelial Cells

ABSTRACT: The goal of this study is to determine the transcriptional response of primary normal human bronchial epithelial (NHBE) cells following interaction with A. fumigatus conidia at 2, 6, and 12 hours in order to examine the infection of airway epithelial cells at multiple time points of co-incubation. After total RNA isolation, the Illumina® HiSeq 2000 next generation sequencing system was used to characterize the transcriptional response, in untreated and A. fumigatus exposed cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE61955 | GEO | 2017/09/21

SECONDARY ACCESSION(S): PRJNA262784

REPOSITORIES: GEO

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Small RNA sequencing of Aspergillus fumigatus

Project description:Aspergillus fumigatus is an important human pathogen and a leading fungal killer. This study aimed to determine the small RNA repertoire of A. fumigatus in conidia and mycelium grown for 24 or 48 hours in liquid culture.

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Gene expression level of Aspergillus fumigatus CEA17_ΔakuBKU80 at three different stages

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Gene expression in Aspergillus fumigatus at different developmental stages

Project description:Microarray analysis was used to compare different divelopmental stages of the filamentous fungi Aspergillus fumigatus af293. Cells were grone for various times and comparisons made between: 1. Dormant conidia (0 hours) and isotropically expanding cells (1hr). 2. Dormant conidia (0 hours) and isotropically expanding cells (2hr) . 3. Dormant conidia (0 hours) and isotropically expanding cells (4hr). 4. Isotropically expanding cells (4hr) and polarity extending cells (6hr). 4. Isotropically expanding cells (4hr) and germ tube (8hr).

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Response of A549 cells treated with Aspergillus fumigatus [WT-GC_vs_PrtT-GC]

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2010-10-29 | E-GEOD-24984 | biostudies-arrayexpress

Response of A549 cells treated with Aspergillus fumigatus [WT-GC_vs_PrtT-GC]

Project description:Response of A549 cells treated with Aspergillus fumigatus wild type germinating conidia (WT_GC) or PrtT protease deficient mutant conidia (PrtT-GC) or inert acrylic 2-4 micron beads (Beads) for 8h Aspergillus fumigatus is the most commonly encountered mold pathogen of humans, predominantly infecting the respiratory system. Colonization and penetration of the lung alveolar epithelium is a key but poorly understood step in the infection process. This study focused on identifying the transcriptional and cell-signaling responses activated in A549 alveolar carcinoma cells incubated in the presence of A. fumigatus wild-type and ΔPrtT protease-deficient germinating conidia and culture filtrates (CF). Microarray analysis of exposed A549 cells identified distinct classes of genes whose expression is altered in the presence of germinating conidia and CF and suggested the involvement of both NFkB and MAPK signaling pathways in mediating the cellular response. Phosphoprotein analysis of A549 cells confirmed that JNK and ERK1/2 are phosphorylated in response to CF treatment in a protease-dependent manner. Inhibition of JNK or ERK1/2 kinase activity substantially decreased CF-induced cell damage, including cell peeling, actin-cytoskeleton damage, and reduction in metabolic activity and necrotic death. These results suggest that inhibition of MAPK-mediated host responses to treatment with A. fumigatus CF decreases cellular damage, a finding with possible clinical implications.

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Aspergillus fumigatus

Project description:transcriptomic analysis of germinating Aspergillus fumigatus conidia

| PRJNA408076 | ENA

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