Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Legionella ITS oligonucleotide microarray

ABSTRACT: In this report, we have developed a rapid oligonucleotide microarray detection technique to identify the most common ten Legionella spp.. The sensitivity of the detection was at 1.0 ng with genomic DNA or 13 CFU/100 mL with Legionella cultures. The microarray detected seven air conditioner-condensed water samples with 100% accuracy, validating the technique as a promising method for applications in basic microbiology, clinical diagnosis, food safety, and epidemiological surveillance. The phylogenetic study based on the ITS has also revealed interestingly that the non-pathogenic L. fairfieldensis is the closest to L. pneumophila than the nine other pathogenic Legionella spp..

ORGANISM(S): Legionella maceachernii Legionella Legionella bozemanae Fluoribacter gormanii Legionella pneumophila Legionella micdadei Legionella anisa Legionella longbeachae Legionella jordanis Fluoribacter dumoffii Legionella fairfieldensis

PROVIDER: GSE61962 | GEO | 2014/10/04

SECONDARY ACCESSION(S): PRJNA263045

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Legionella pneumophila small RNAs and deletion of 6S RNA

Project description:This SuperSeries is composed of the following subset Series: GSE19196: Detection of predicted small RNA of Legionella pneumophila GSE19200: Gene affected by deletion of 6S RNA in post-exponential phase Refer to individual Series

2010-06-24 | E-GEOD-19451 | biostudies-arrayexpress

Legionella pneumophila transcriptomic response to CuO nanoparticles

Project description:Legionella pneumophila was exposed for 3h to 160 ug/ml of CuO nanoparticle. Untreated samples are used as the control condition.

2013-03-04 | GSE41966 | GEO

Legionella pneumophila transcriptome during intracellular multiplication in human macrophages

Project description:Legionella pneumophila is the causative agent of Legionnaires’ disease, an acute pulmonary infection. L. pneumophila is able to infect and multiply in both phagocytic protozoan, such as Acanthamoeba castellanii, and mammalian professional phagocytes. The best-known virulence determinant used by L. pneumophila to infect host cells is a Type IVb translocation system named Icm/Dot, which is used to modify the host cell functions to the benefit of the bacteria. To date the Icm/Dot systeme is known to translocate more than 100 effectors. While the transcriptional response of Legionella to the intracellular environement of A. castelannii as already been investigated, much less is known of how Legionella reacts transcriptionnally inside human macrophages. In this study, the transcriptome of L. pneumophila was monitored during exponential and post-exponential phase in rich AYE broth and during infection of human cultured macrophages by using microarray and a RNA amplification procedure called SCOTS to allow for the study of conditions of low bacterial loads. Among the genes induced intracellularly are those involved in amino acid synthesis pathway leading to L-arginine, L-histidne and L-proline as well as many transport system involved in amino acid and iron uptake. The Icm/Dot systems is not differentially expressed inside cells compare to the E phase control but the effectors are strongly induced. The intracellular transcriptome was further used to identify putative new Icm/Dot effectors and translocation was show to occur for 3 of them. This study provides a comprehensive view of how L. pneumophila react to the human macrophages intracellular environment.

2011-01-01 | GSE23029 | GEO

small RNA profiling of human blood derived macrophages upon infection with Legionella Pneumophila

Project description:Legionella pneumophila (corby) infected blood derived macrophages are infected in a time course experiment (24& 48hours)

2019-01-25 | GSE125559 | GEO

Legionella pneumophila transcriptomic response of hfq mutant during post-exponential phase

Project description:Legionella pneumophila wild-type and hfq mutant were grown to post-exponential. The wild-type strain is used as the control.

2013-05-21 | GSE42905 | GEO

Transcription profiling of Legionella pneumophila wild type vs fleR mutant at OD 3.3

Project description:Comparison of gene expressions of Legionella pneumophila wide types and fleR mutants. Liquid culture (60 ml) BCYE, shaking, 37 ¡C. GenePix Pro for quantification with parameters: Background and Measure. BloDAP and R: adjustments - Bonferroni and Benjamini & Yekutieli; normalization - global loess; transformation - log; test - student test with varmixt; filtering - spot saturation detection. Axon for scanning. Contact: Christian Weber and Tobias Sahr

2009-11-24 | E-MTAB-163 | biostudies-arrayexpress

Strand specific sequencing illustrates the complex transcriptional response of Legionella pneumophila during infection

Project description:Legionella pneumophila is a Gram-negative facultative intracellular human pathogen with a distinct biphasic lifestyle. One of its primary environmental hosts in the free-living amoeba Acanthamoeba castellanii and its infection by L. pneumophila mimics that seen in human macrophages. Here we present analysis of strand specific sequencing of the transcriptional response of L. pneumophila in broth growth and in infection of A. castellanii.

2011-02-11 | GSE27232 | GEO

Legionella pneumophila

Project description:Legionella pneumophila

| PRJNA526503 | ENA

Legionella pneumophila cells after sub-lethal heat shock

Project description:Legionella pneumophila Philadelphia-1 strain was grown to stationary phase in AYE broth and starved in freshwater for 2 hours and RNA was harvested with or without sublethal heat shock via immersion in a 55 degree C hot water bath for 5 minutes

2022-02-15 | GSE196713 | GEO

Legionella pneumophila proteome

Project description:Legionella pneumophila cells were harvested during exponential growth (RP) and stationary growth (TP). VBNC cells were also anylzed. Protein subfractions were studied.

2015-11-13 | PXD001926 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data