Rat Semi-Circular Canal Duct Gene Expression Studies
Ontology highlight
ABSTRACT:
ORGANISM(S): Rattus norvegicus
PROVIDER: GSE6197 | GEO | 2010/04/14
SECONDARY ACCESSION(S): PRJNA100683
REPOSITORIES: GEO
Similar Datasets
Project description:The goal was to screen for the expressed genes in Semi-Circular Canal Duct (SCCD) that are related to ion transport and its regulation. The objective was to discover which genes changed expression levels in response to glucocorticoids. Keywords: drug response Primary cultures were incubated for 24 hours in the presence or absence of 100 nM dexamethasone. Four independent cultures from each group were processed for total RNA and submitted for gene array analysis. Although two Samples out of four in each group were done at different time points, we found that there was not much variation between the datasets (by cluster analysis).
2010-04-28 | E-GEOD-6197 | biostudies-arrayexpress
Project description:Rat Semi-Circular Canal Duct Gene Expression Studies
| PRJNA100683 | ENA
Project description:PURPOSE:Adolescent idiopathic scoliosis (AIS) is a three-dimensional spinal structural deformity that occurs in otherwise normal individuals. Although curve progression and severity vary amongst individuals, AIS can lead to significant cosmetic and functional deformity. AIS etiology has been determined to be genetic, however, exact genetic and biological processes underlying this disorder remain unknown. Vestibular structure and function have potentially been related to the etiopathogenesis of AIS. Here, we aimed to characterize the anatomy of the semicircular canals (SCC) within the vestibular system through a novel approach utilizing T2-weighted magnetic resonance images (MRI). METHODS:Three dimensional, MRI-based models of the SCCs were generated from AIS subjects (n = 20) and healthy control subjects (n = 19). Linear mixed models were used to compare SCC morphological measurements in the two groups. We compared side-to-side differences in the SCC measurements between groups (group*side interaction). RESULTS:Side-to-side differences in the lateral SCC were different between the two groups [false discovery rate adjusted p-value: 0.0107]. Orientation of right versus left lateral SCC was significantly different in the AIS group compared to the control group [mean side-to-side difference: -4.1°, 95% CI: -6.4° to -1.7°]. Overall, among subjects in the AIS group, the left lateral SCC tended to be oriented in a more horizontal position than subjects in the control group. SIGNIFICANCE:Asymmetry within the SCCs of the vestibular system of individuals with AIS potentially results in abnormal efferent activity to postural muscles. Consequences of this muscular activity during periods of rapid growth, which often coincides with AIS onset and progression, warrant consideration.
| S-EPMC7190182 | biostudies-literature
Project description:External auditory canal squamous cell carcinoma (EACC) is very rare, only accounting for two thousandth of the head and neck cancer. However, the development mechanism of EACC remains unknown. By using gene expression microarray analysis, we aimed to find differentially expressed genes involved in ESCC development. We identified a wide spectrum of molecular signatures in EACC, including mRNA and lncRNA. The present study systematically analyzed the expression of mRNA and lncRNA in squamous cell carcinoma of the external auditory canal and normal external auditory canal tissues. We detect the transcriptomic changes between squamous cell carcinoma of the external auditory canal and normal external auditory canal tissues to identify potential tumor biomarkers in squamous cell carcinoma of the external auditory canal.
2018-02-14 | GSE98912 | GEO
Project description:Etiologically linked to HPV infection, malignancies of the anal canal have substantially increased in incidence over the last 20 years. Although most anal squamous cell carcinomas (SCC) respond well to chemoradiotherapy, for undetermined reasons, a subgroup of patients experience a poor outcome. Despite cumulative efforts for discovering independent predictors for overall survival, both nodal status and tumor size are still the only reliable factors predicting patient outcome. In the present study, we correlated both proteomic signatures and clinicopathological features of neoplastic lesions arising from two distinct portions of the anal canal: the lower part (squamous zone) and the more proximal anal transitional zone. Although microdissected cancer cells appeared indistinguishable by morphology (squamous phenotype), unsupervised clustering analysis of the whole proteome significantly highlighted the heterogeneity that exists within anal canal tumors. More importantly, two region-specific subtypes of SCC were revealed. The expression profile (sensitivity/specificity) of several selected biomarkers (keratin filaments) further confirmed the subclassification of anal (pre)cancers based on their cellular origin. Less commonly detected compared to their counterparts located in the squamous mucosa, SCC originating in the transitional zone displayed more frequently a poor or basaloid differentiation and were significantly correlated with reduced disease-free and overall survivals. Taken together, we present for the first time direct evidence that anal canal SCC comprises two distinct entities with different cells of origin, proteomic signatures and survival rates. This study forms the basis for a novel dualistic classification of anal carcinoma with implications for management, outcome expectations and possibly therapeutic approaches.
2016-12-20 | PXD004859 | Pride
Project description:Proteomic analysis of Amphibalanus amphitrite longitudinal canal tissue in comparison to sub-mantle/reproductive tissue to ascertain functional similarity.
2018-11-14 | PXD010743 | Pride
Project description:The semi-circular canals in the inner ear sense head rotations. It is widely recognised that the anatomy of the semi-circular canals is often adapted to the species-specific agility, in order to provide the necessary sensitivity. Based on research on mammals, the ellipticity of the semi-circular canal was so far considered as a non-important factor herein. A dataset of 125 squamate species and 156 mammalian species, now shows that the posterior semi-circular canal of squamates is much more elliptical (eccentricities ranging between 0.76 and 0.94) than that of mammals (eccentricities ranging between 0 and 0.71). Fluid-Structure Interaction computer models show that the effect of the ellipticity on sensitivity is strongest in small semi-circular canals. This new insight indicates that the high ellipticity in squamates leads to a severe reduction in sensitivity of up to 45%. In mammals, on the other hand, the reduction in sensitivity is limited to 13%, which is consistent with previous literature that found a limited effect of semi-circular canal ellipticity in mammals. Further, there is a strongly negative correlation between semi-circular canal size and eccentricity in squamates, which is absent in mammals. Hence, the smallest squamates have the most elliptical semi-circular canals. In general, the smaller the semi-circular canal, the less sensitive it is. Therefore, the highly elliptical squamate canals are probably the result of fitting the largest possible canal in small and flat head. Miniaturising the canals while maintaining a circular shape would reduce the sensitivity by another 73% compared to the highly elliptical canals.
| S-EPMC6848070 | biostudies-literature
Project description:The goal was to screen for the expressed genes in Reissner's membrane (RM) that are related to ion transport and its regulation. The objectives were 1) to determine whether short-term incubation altered the transcriptome and 2 ) to discover which genes changed expression levels in response to glucocorticoids. Keywords: drug response
2009-03-21 | GSE6196 | GEO