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PiRNAs and transgenerational RNA interference protect essential genes from RNA silencing in C. elegans

ABSTRACT: This data series contains small RNA high-throughput sequencing data derived from cell lysates and FLAG::HRDE-1 immunoprecipitates (IP) after resetting RNAi in the presence or absence of piRNAs in C. elegans.

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE63114 | GEO | 2015/06/30

SECONDARY ACCESSION(S): PRJNA266654

REPOSITORIES: GEO

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piRNAs and transgenerational RNA interference protect essential genes from RNA silencing in C. elegans

Project description:This data series contains small RNA high-throughput sequencing data derived from cell lysates and FLAG::HRDE-1 immunoprecipitates (IP) after resetting RNAi in the presence or absence of piRNAs in C. elegans. Small RNAs were isolated from adult wild type and mutant or transgenic C. elegans and subjected to Illumina HiSeq sequencing. The series contains fastq and tab-separated files for 10 libraries.

2015-06-30 | E-GEOD-63114 | biostudies-arrayexpress

P granules protect RNA interference genes from silencing by piRNAs

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P Granules Protect RNA Interference Genes from Silencing by piRNAs.

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P granules protect RNA interference genes from silencing by piRNAs

Project description:This SuperSeries is composed of the SubSeries listed below.

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Project description:To explore the roles of piRNAs in regulating 22G-RNA production and transgenerational gene silencing, we used RNA-seq to assess changes in small RNA and mRNA levels in piwi/prg-1 and mutator mutants at an early generation and again at a late generation. We identified that in the absence of piRNAs, many genes gain 22G-RNAs which in turn guide transgenerational gene silencing.

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