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Analysis of the transcriptional response of Candida parapsilosis following exposure to farnesol

ABSTRACT: We used the results of a genome sequence survey to design and manufacture partial genomic microarrays that were applied to determining the transcriptional response of C. parapsilosis to the presence of exogenous farnesol. Keywords: drug response, farnesol,

ORGANISM(S): Candida parapsilosis

PROVIDER: GSE6318 | GEO | 2007/03/01

SECONDARY ACCESSION(S): PRJNA100531

REPOSITORIES: GEO

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Analysis of the transcriptional response of Candida parapsilosis following exposure to farnesol

Project description:We used the results of a genome sequence survey to design and manufacture partial genomic microarrays that were applied to determining the transcriptional response of C. parapsilosis to the presence of exogenous farnesol. Keywords: drug response, farnesol, Transcriptional profiling was performed following exposure to 50 microM of farnesol compare to the control experiment (no treatment). Six independent experiment comparisons (including 3 dye swaps) were performed.

2010-06-11 | E-GEOD-6318 | biostudies-arrayexpress

Candida parapsilosis

Project description:Analysis of the transcriptional response of Candida parapsilosis following exposure to farnesol

| PRJNA100531 | ENA

Comparative transcriptional analysis of Candida auris biofilms following farnesol and tyrosol treatment

Project description:Candida auris is frequently associated with biofilm-related invasive infections. The resistant profile of these biofilms necessitates innovative therapeutic options, where quorum sensing may be a potential target. Farnesol and tyrosol are two fungal quorum-sensing molecules with antifungal effects at supraphysiological concentrations. Here, we performed genome-wide transcript profiling with C. auris biofilms following farnesol or tyrosol exposure using transcriptome sequencing (RNA-Seq). Since transition metals play a central role in fungal virulence and biofilm formation, levels of intracellular calcium, magnesium, and iron were determined following farnesol or tyrosol treatment using inductively coupled plasma optical emission spectrometry. Farnesol caused an 89.9% and 73.8% significant reduction in the calcium and magnesium content, respectively, whereas tyrosol resulted in 82.6%, 76.6%, and 81.2% decrease in the calcium, magnesium, and iron content, respectively, compared to the control. Genes involved in biofilm events, glycolysis, ergosterol biosynthesis, fatty acid oxidation, iron metabolism, and autophagy were primarily affected in treated cells. To prove ergosterol quorum-sensing molecule interactions, microdilution-based susceptibility testing was performed, where the complexation of farnesol, but not tyrosol, with ergosterol was impeded in the presence of exogenous ergosterol, resulting in a minimum inhibitory concentration increase in the quorum-sensing molecules. This study revealed several farnesol- and tyrosol-specific responses, which will contribute to the development of alternative therapies against C. auris biofilms.

2023-12-11 | GSE233427 | GEO

Transcriptional profiling of the Candida auris response to exogenous farnesol exposure

Project description:Transcriptional profiling of the Candida auris response to exogenous farnesol exposure

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Candida parapsilosis

Project description:Transcriptional response of an azole-resistant Candida parapsilosis isolate [voriconazole].

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Project description:Transcriptional response of an azole-resistant Candida parapsilosis isolate [fluconazole].

| PRJNA141841 | ENA

Candida parapsilosis

Project description:Transcriptional response of an azole-resistant Candida parapsilosis isolate [posaconazole].

| PRJNA141837 | ENA

Candida parapsilosis

Project description:Candida parapsilosis WGS

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Comparative phenotypic analysis of the major fungal pathogens Candida parapsilosis and Candida albicans

Project description:Abstract: Candida parapsilosis and Candida albicans are human fungal pathogens that belong to the CUG clade in the Saccharomycotina. In contrast to C. albicans, relatively little is known about the virulence properties of C. parapsilosis, a pathogen particularly associated with infections of premature neonates. We describe here the construction of >200 C. parapsilosis strains carrying double allele deletions of transcription factors, protein kinases and species-specific genes. Two independent deletions were constructed for each target gene. Growth in > 40 conditions was tested, including carbon source, temperature, and the presence of antifungal drugs. The phenotypes were compared to C. albicans strains with deletions of orthologous transcription factors. We found that many phenotypes are shared between the two species, such as the role of Upc2 as a regulator of azole resistance. Others are unique. For example, Cph2 plays a role in the hypoxic response in C. parapsilosis and not in C. albicans. We found extensive divergence between the biofilm regulators of the two species. We identified 7 transcription factors and one protein kinase that are required for biofilm development in C. parapsilosis. Only three (Efg1, Bcr1, and Ace2) have similar effects on C. albicans biofilms, whereas Cph2, Czf1, Gzf3 and Ume6 have major roles in C. parapsilosis only. In addition, two transcription factors (Brg1 and Tec1) with well-characterized roles in biofilm formation in C. albicans do not have the same function in C. parapsilosis. We also compared the transcription profile of C. parapsilosis and C. albicans biofilms. Our analysis suggests the processes shared between the two species are predominantly metabolic.

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Hypoxia and biofilms in Candida parapsilosis

Project description:This SuperSeries is composed of the following subset Series: GSE13717: Transcriptional profile of Candida parapsilosis in SD media GSE13722: Transcriptional response of Candida parapsilosis in low oxygen (hypoxic) conditions in SD media Refer to individual Series

2010-05-06 | E-GEOD-13832 | biostudies-arrayexpress

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