Project description:Transcriptional profiling of Bmi1 mutant dental epithelia including the stem cell compartment to determine which genes are upregulated in response to loss of Bmi1.
Project description:Transcriptional profiling of Bmi1 mutant dental epithelia including the stem cell compartment to determine which genes are upregulated in response to loss of Bmi1. Two condition experiment: dental epithelia homozygous null for Bmi1 and WT dental epithelia. 4 replicates each
Project description:Identification of BMI1, RYBP and H2AK119UB interactome in Glioblastoma (GBM) to elucidate BMI1 roles independent of the PRC1-complex in GBM.
Project description:To facilitate comparative genomic analyses of human fetal and adult cells undergoing erythropoiesis, we employed a serum-free two-phase liquid culture system to expand and differentiate primary human CD34+ hematopoietic stem/progenitor cells (HSPCs) ex vivo. In this experimental context, highly enriched populations of stage-matched, differentiating, primary proerythroblasts (ProEs) were generated. We selected four time points (day 0, CD34+ HSPCs; day 3, 5, and 7, differentiating ProEs) that represented similar stages differentiation for gene expression profiling using microarrays.
Project description:Neural stem cells were isolated from embryonic (E18) cortex and from adult mouse subventricular zone and transduced with a Bmi1-overexpressing lentiviral vector or an empty vector control. Cells were grown as neurospheres (in non-adherent culture conditions) for two passages for eNSCs and three passages for aNSCs and RNA purified (after three weeks for eNSCs and four weeks for aNSCs).
Project description:Bmi1 is a component of polycomb repressive complex 1 and its role in the inheritance of the stemness of adult somatic stem cells has been well characterized. Bmi1 maintains the self-renewal capacity of adult stem cells, at least partially, by repressing the Ink4a/Arf locus that encodes a cyclin-dependent kinase inhibitor, p16Ink4a, and a tumor suppressor, p19Arf 14. Deletion of both Ink4a and Arf in Bmi1-deficient mice substantially restored the defective self-renewal capacity of HSCs and neural stem cells. Purified CMP from BM of recipient mice repopulated with wild-type, Ink4a-/-Arf-/-, and Bmi1-/- Ink4a-/-Arf-/- BM cells were subjected to RNA extraction and hybridization on Affymetrix microarrays.
Project description:In order to identify the key genes for self-renwal abiity of innate immune B-1a cells, we have gene expression analysis using Aglinant Whole Mouse gGenome Oligo Microarrays performed by Miltenyi Biottec. We isolated peritoneal B-1a cells from wild type mice and Bmi1 kockout (KO) mice that lost self-renewal ability of B-1a cells, and submitted purified RNA from each samples to Miltenyi.