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Expression data for human nasopharyngeal carcinoma cell line HK1 stimulated with EGF or TGF-β recombinant protein.

ABSTRACT: As regulators in gene expression, microRNAs take part in most biological process including cell differentiation, apoptosis, cell cycle and epithelial-to-mesenchymal transition (EMT). In order to evaluate their roles during the growth factors-induced EMT process, microRNA expression profile changes induced by EGF or TGF-β treatment on nasopharyngeal carcinoma cell line HK-1 cells were analyzed by means of the Affymetrix genome wide microarray system.

ORGANISM(S): synthetic construct Homo sapiens

PROVIDER: GSE64281 | GEO | 2015/12/17

SECONDARY ACCESSION(S): PRJNA270629

REPOSITORIES: GEO

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Expression data for human nasopharyngeal carcinoma cell line HK1 stimulated with EGF or TGF-Î² recombinant protein.

Project description:As regulators in gene expression, microRNAs take part in most biological process including cell differentiation, apoptosis, cell cycle and epithelial-to-mesenchymal transition (EMT). In order to evaluate their roles during the growth factors-induced EMT process, microRNA expression profile changes induced by EGF or TGF-Î² treatment on nasopharyngeal carcinoma cell line HK-1 cells were analyzed by means of the Affymetrix genome wide microarray system. Human nasopharyngeal carcinoma cell line HK-1 was starved for 12 hours before stimulation with EGF (50 ng/mL) or TGF-Î² (10ng/ml) or Control (PBS) for 36 hours in RPMI-1640 medium (with 1% serum) to establish the in vitro model of EMT. Total RNA were extracted and detected by AffymetrixÂ® GeneChipÂ® miRNA Arrays.

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Gene Transcriptional Variation of Hepatic Progenitor Cells after TGF- 1-Induced EMT Transition and EGF-Induced MET Reversion

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Microarray analysis of differentially expressed genes in bicalein-treated Nasopharyngeal carcinoma (HK-1) cell line

Project description:Baicalein is a bioactive compound that is found in an herbal plant, Oroxylum indicum, grown in Southeast Asia including Thailand. There are several reports investigating on the bioactivities of baicalein including an anticancer activity. However, there is no report on the anticancer activity of the baicalein in nasopharyngeal carcinoma. Thus, the aim of this study is to identify differentially expressed genes (DEGs) that are targets of baicalein in order to reveal cellular pathways and molecular mechanisms of the baicalein in nasopharyngeal carcinoma. In this study, NPC HK-1 cells were used as an NPC representative and treated with 10 and 20 µM baicalein for 24 hours before collecting cells for whole transcriptome profiles. The results revealed that a number of cellular pathways, based on KEGG enrichment analysis, are affected baicalein treatment such as focal adhesion and PI3K-Akt signaling pathway which were also phenotypically related to cell migration and invasion assays. Changes in transcriptome profiles of baicalein-treated HK-1 cells would provide insight on baicalein activity in NPC.

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