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Genome-wide mapping of DNA hydroxymethylation in osteoarthritic chondrocytes [methylation]

ABSTRACT: Examination of the genome-wide distribution of 5hmC in osteoarthritic chondrocytes compared to normal chondrocytes in order to elucidate the effect on OA-specific gene expression. 5hmC-sequencing was performed and data was compared with microarray gene expression data to identify genes with differential expression between normal and OA chondrocytes that are potentially under epigenetic regulation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE64393 | GEO | 2015/06/30

SECONDARY ACCESSION(S): PRJNA270885

REPOSITORIES: GEO

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Genome-wide mapping of DNA hydroxymethylation in osteoarthritic chondrocytes [expression]

2015-06-30 | GSE64394 | GEO

Genome-wide mapping of DNA hydroxymethylation in osteoarthritic chondrocytes [methylation]

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Genome-wide mapping of DNA hydroxymethylation in osteoarthritic chondrocytes [expression]

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2015-06-30 | E-GEOD-64394 | biostudies-arrayexpress

Ribosomal RNA 2’-O-methylation profile of human articular chondrocytes exposed to an osteoarthritic environment

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Project description:Human chondrocytes were obtained from the cartilage of femoral condyles and tibial plateaus from individuals within 12 hours of death (normal) and from patients undergoing total knee arthroplasty (osteoarthritic, OA). The chondrocytes were released from the cartilage, seeded at high density, and cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated fetal bovine serum and an antibiotic mixture (100 units/ml penicillin base and 100 μg/ml streptomycin base) at 37°C in a humidified atmosphere of 5% CO2/95% air. Total RNA was extracted from the primary chondrocytes and processed for qPCR according to the manufacturer's instructions (SA Biosciences RT2 Profiler PCR Array PAHS-089Z).

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Genome-wide mapping of DNA hydroxymethylation in osteoarthritic chondrocytes

Project description:Genome-wide mapping of DNA hydroxymethylation in osteoarthritic chondrocytes

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Real-time quantitative PCR analysis of UPR genes in human osteoarthritic chondrocytes specimens treated with thapsigargin compared to the untreated (control) chondrocytes

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