Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Exon-Intron circular RNAs regulate transcription in the nucleus

ABSTRACT: We report the high-throughput sequencing of Pol II

ORGANISM(S): Homo sapiens

PROVIDER: GSE64443 | GEO | 2015/02/01

SECONDARY ACCESSION(S): PRJNA271017

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Exon-Intron circular RNAs regulate transcription in the nucleus

Project description:We report the high-throughput sequencing of Pol II One sample of IgG and Pol II

2015-02-01 | E-GEOD-64443 | biostudies-arrayexpress

RECQL5 and transcription-coupled DNA repair complex coordinate to regulate transcription elongation

Project description:The RECQL5 helicase is crucial for maintaining genome stability and functions at the intersection of transcription, replication, and DNA repair. RECQL5 associates with RNA15 polymerase II (Pol II) to modulate transcription elongation, yet the underlying mechanism remains unclear. Here, we report cryo-electron microscopy structures of human RECQL5 bound to multiple transcription elongation complexes. Combined with biochemical analysis, we identified an a-helix of RECQL5 responsible for Pol II binding and inhibition of transcription elongation. We further reveal that the transcription-coupled DNA repair (TCR) complex20 reactivates the transcription of RECQL5-inhibited Pol II through concerted actions of its translocase activity and competition with the RECQL5 helix for engaging Pol II. Our results suggest a model in which RECQL5 and TCR coordinately regulate the transcription elongation rate to ensure transcription efficiency while maintaining genome stability.

2025-09-22 | PXD062042 | Pride

Sensing Self and Foreign Circular RNAs by Intron Identity.

Project description: Not available

| S-EPMC5610545 | biostudies-literature

Developing an enhanced chimeric permuted intron-exon system for circular RNA therapeutics.

Project description: Not available

| S-EPMC11426236 | biostudies-literature

Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture [PRO-seq]

Project description:Genome binding/occupancy profiling by high throughput sequencing ｜ Expression profiling by high throughput sequencing | Other Mammalian nuclei contain Pol I, Pol II, and Pol III. However, to what extent and how they are cross-regulated remains elusive. Here, we performed orthogonal multi-omics profiling after acute degradation of the largest subunits of Pol I, Pol II, and Pol III, and showed that they mainly affect specific genes. In contrast, the loss of Pol I or Pol II causes few changes for other RNA polymerases and confirms those known. The changes of Pol II transcription after Pol III depletion are the largest among all the cross-regulatory types. Meta-analyses reveal that Pol III depletion increases nucleosome positioning, reduces the FACT complex occupancy, and perturbs Pol II elongation for nearby mRNA genes. Furthermore, the nucleosome positioning changes also underpinning the Pol II effects on Pol III-mediated tRNA transcription. Our results suggest that Pol III works together with Pol II to coordinate their transcription activities by maintaining local chromatin architecture.

2022-11-07 | GSE181699 | GEO

Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture [ChIP-seq]

2022-11-07 | GSE181698 | GEO

Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture [ChAR-seq]

2022-11-07 | GSE181697 | GEO

Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture [ATAC-seq]

2022-11-07 | GSE181696 | GEO

Accumulation of Stable Full-Length Circular Group I Intron RNAs during Heat-Shock.

Project description: Not available

| S-EPMC6274462 | biostudies-literature

Allosteric transcription stimulation by super elongation complex

Project description:The super elongation complex (SEC) contains the positive transcription elongation factor b (P-TEFb) and a subcomplex, ELL2-EAF1, which stimulates transcription elongation by RNA polymerase II (Pol II). Here we report the cryo-EM structure of ELL2-EAF1 bound to a Pol II elongation complex at 2.8 Å resolution. The ELL2-EAF1 dimerization module directly binds the Pol II lobe, explaining how SEC delivers P-TEFb to Pol II. The same site on the lobe also binds the initiation factor TFIIF, consistent with SEC binding only after the transition from transcription initiation to elongation. Structure-guided functional analysis shows that elongation stimulation requires the dimerization module and an ELL2 protein linker that tethers this module to the Pol II protrusion. Our results show that SEC stimulates elongation allosterically and indicate that this stimulation involves stabilization of a further closed conformation of the Pol II active center cleft.

2021-07-15 | PXD024457 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data