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Genome-wide identification of Bacillus subtilis Zur-binding sites.

ABSTRACT: The transcriptional factor Zur plays a key role in regulating zinc homeostasis in Bacillus subtilis. The genomic sites bound by Zur were mapped using a chromatine immunoprecipitation approach. This allowed the identification of 80 inter- and intragenic chromosomal sites bound by Zur.

ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168

PROVIDER: GSE64671 | GEO | 2015/01/06

SECONDARY ACCESSION(S): PRJNA271606

REPOSITORIES: GEO

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Genome-wide identification of Bacillus subtilis Zur-binding sites.

Project description:The transcriptional factor Zur plays a key role in regulating zinc homeostasis in Bacillus subtilis. The genomic sites bound by Zur were mapped using a chromatine immunoprecipitation approach. This allowed the identification of 80 inter- and intragenic chromosomal sites bound by Zur. This data set contains 2 samples. Immunoprecipitated (IP) DNA from Bacillus subtilis BSAS36 strain (BSB1, a tryptophan-prototrophic derivative 168 strain expressing a SPA-tagged Zur protein) were extracted from bacterial cells in the exponential growth phase. IP and whole-cell extract DNA were hybridized on tiling array to generate ChIP-on-chip profiles. Two biological replicates were anlyzed.

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