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Global re-wiring of p53 transcription regulation by the hepatitis B virus X (HBx) protein [ChIP-Seq]

ABSTRACT: As HBx has been reported to interact with p53 and alter the recruitment of p53 to its binding sites, we obtained a comprehensive genome-wide profile of deregulated p53 transcription complex-DNA binding by the HBx protein using massively parallel deep sequencing coupled to p53 chromatin immunoprecipitation (ChIP-Seq) on HBx-expressing and control HepG2 liver cell culture model system.

ORGANISM(S): Homo sapiens

PROVIDER: GSE64877 | GEO | 2016/06/22

SECONDARY ACCESSION(S): PRJNA272433

REPOSITORIES: GEO

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Global re-wiring of p53 transcription regulation by the hepatitis B virus X (HBx) protein [Agilent]

Project description:As HBx has been reported to interact with p53 and alter the recruitment of p53 to its binding sites, we would like to identify p53-associated genes that were deregulated by HBx. We employed whole genome microarray expression profiling to identify genes that were deregulated by HBx protein.

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Global re-wiring of p53 transcription regulation by the hepatitis B virus X (HBx) protein

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Global re-wiring of p53 transcription regulation by the hepatitis B virus X protein.

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Obesity synergizes with HBx, Src and p53 mutation accelerate hepatocarcinogenesis [Microarray]

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Project description:Hepatitis B virus (HBV) has been clearly recognized as an etiological factor for hepatocellular carcinoma (HCC). HBV encodes the potentially oncogenic HBx protein. We aimed to elucidate the molecular mechanism of HCC caused by HBx and to discover the biomarker related to HCC by HBx. Three experimental groups, 3, 9 and 13 month aged HBx Tg mice and age matched normal wild type B6 mouse which have same background of HBx Tg mice were used to find differentially expressed genes during HCC. Keywords: Genetic modification 3-month-old, 9-month-old, 13-month-old wild type B6 mice vs 3-month-old, 9-month-old, 13-month-old HBx transfected mice; Biological replicates at each timepoint; 9 controls vs 9 HBx-mice

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