Transcriptomics

Dataset Information

0

Polycomb Underlies Transcriptional Heterogeneity in Lineage Priming of Embryonic Stem Cells [gene expression]


ABSTRACT: Quantitation of polyA mRNA levels in subpopulations of the HexVenus reporter (clone HV5.1) mouse ES cell line growing under self-renewing conditions. Subpopulations were identified and isolated based on the expression of the ES cell surface marker SSEA 1 and the expression of the venus protein. At approximately 70% confluence, cells were trypsinised, resuspended in FACs buffer (10%FCS in PBS) and incubated with a mouse monoclonal antibody to SSEA 1 (MC-480, Developmental Hybridoma Studies Bank, University of Iowa). Cells were then incubated with an Alexa-647 conjugated anti-mouse IgM antibody (Invitrogen) and subpopulations were fractionated by flow cytometry. The aim was to identify genes which are differentially expressed between FACS-sorted HV-S+ and HV+S+ primed mESC populations.

ORGANISM(S): Mus musculus

PROVIDER: GSE64939 | GEO | 2016/10/11

SECONDARY ACCESSION(S): PRJNA272538

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2016-10-11 | GSE84576 | GEO
2016-10-11 | GSE65288 | GEO
2010-06-08 | GSE13472 | GEO
2022-01-03 | GSE58503 | GEO
2015-05-26 | GSE54595 | GEO
2015-05-26 | E-GEOD-54595 | biostudies-arrayexpress
2015-05-26 | E-GEOD-48384 | biostudies-arrayexpress
2015-05-26 | GSE48384 | GEO
2024-07-16 | PXD045076 | Pride
2007-02-28 | E-GEOD-5909 | biostudies-arrayexpress