Beauveria bassiana MBF 1 (BbMBF1) regulating genes involved in fungal resistance to oxidative stress
Ontology highlight
ABSTRACT: Gene BbMBF1 playes an essential role in fungal response to oxidative stress, a determinant to the biocontrol potential of entomopathogenic fungi. The genome-wide exprssion analysis involved in stress response was analyzed by using high throughput sequencing (RNA-Seq). Our transcriptional profiles revealed that numerous differentially expressed genes (DEGs), of which involved in metabolism, cell transport and cell rescue, were significantly controlled by BbMBF1 during response to oxidative stress.
Project description:Gene BbSwi6 playes an essential role in asexual differentiation, a determinant to the biocontrol potential of entomopathogenic fungi. The genome-wide exprssion analysis involved in fungal development was analyzed by using high throughput sequencing (RNA-Seq).
Project description:Gene BbGlc8 playes an essential role in conidation, a determinant to the biocontrol potential of entomopathogenic fungi. The genome-wide exprssion analysis involved in fungal conidiation was analyzed by using high throughput sequencing (RNA-Seq). Our transcriptional profiles revealed that numerous differentially expressed genes (DEGs), of which involved in metabolism, cell transport and cell rescue, were significantly involved in deveopment of fungal conida.
Project description:Gene BbMbp1 playes an essential role in asexual differentiation, a determinant to the biocontrol potential of entomopathogenic fungi. The genome-wide exprssion analysis involved in fungal development was analyzed by using high throughput sequencing (RNA-Seq). Our transcriptional profiles revealed that numerous differentially expressed genes (DEGs), of which involved in metabolism, cell transport and cell rescue, were significantly involved in asexual deveopment of B. bassiana.
Project description:Gene BbSNF1 playes an essential role in mediating metabolism and cell development during conidation, a determinant to the biocontrol potential of entomopathogenic fungi. The genome-wide exprssion analysis involved in fungal conidiation was analyzed by using high throughput sequencing (RNA-Seq). Our transcriptional profiles revealed that numerous differentially expressed genes (DEGs), of which involved in metabolism, cell transport and cell rescue, were significantly involved in deveopment of fungal conida. .
Project description:Gene BbSNF1 playes an essential role in mediating metabolism and cell development during conidation, a determinant to the biocontrol potential of entomopathogenic fungi. The genome-wide exprssion analysis involved in fungal conidiation was analyzed by using high throughput sequencing (RNA-Seq). Our transcriptional profiles revealed that numerous differentially expressed genes (DEGs), of which involved in metabolism, cell transport and cell rescue, were significantly involved in deveopment of fungal conida. . Total RNA obtained from BbSNF1 disruption mutant were compared to that of wild type strain (WT) grown on SDA plates (Glucose 4%, Peptone 1%, and Yeast extract 1% plus 1.5% Agar).
Project description:Gene BbabaA and BbbrlA play an essential role in conidation, a determinant to the biocontrol potential of entomopathogenic fungi. The genome-wide exprssion analysis involved in fungal conidiation was analyzed by using high throughput sequencing (RNA-Seq). Our transcriptional profiles revealed that numerous differentially expressed genes (DEGs), of which involved in metabolism, cell transport and cell rescue, were significantly involved in deveopment of fungal conida.
Project description:Beauveria basiana is a well-known filamentous entomopathogenic fungus. Oxidation tolerance is an important determinant to fungal pathogenicity and biocontrol potential. B. bassiana transcriptional co-activator multiprotein bridging factor 1 (BbMBF1) contributes to fungal resistance to the oxidative stress. The interactome of BbMBF1 was revealed by the qualitative proteomic analysis integrated with the immuno-precipitation. This study is sought to unveil the comprehensive protein interaction of BbMBF1 and isolate the BbMBF1-mediated transcription factor under oxidative stress.
Project description:Insect cellular immunue plays an important role in recognition and eliminating the intruding entomopathogenic fungi. The genome-wide exprssion analysis involved in the cellular responses was analyzed by using high throughput sequencing (RNA-Seq). Our transcriptional profiles revealed that numerous differentially expressed genes (DEGs), of which involved in metabolism, immune system, cellular processing and so on, were significantly involved in the responses of G. mellonnella hemocytes.
Project description:Gene BbHapX plays an essential role in maturation of conidia, a dorminant spore type for the biocontrol potential of entomopathogenic fungi. The genome-wide expression analysis involved in fungal development was analyzed by using high throughput sequencing (RNA-Seq). Our transcriptional profiles revealed that numerous differentially expressed genes (DEGs), of which involved in metabolism, cell transport and cell rescue, were significantly involved in asexual development of B. bassiana.
Project description:Ssk1-type response regulator proteins are the core elements of histidine-to-aspartate systems that mediate fungal stress tolerance, a determinant to the biocontrol potential of fungal entomopathogens. We characterized for the first time the functions of Beauveria bassiana Ssk1 (Bbssk1) by analyzing multi-phenotypic changes in DBbssk1 and differentially expressed genes (DEGs) in the digital gene expression (DGE) libraries of DBbssk1 and wild-type constructed under osmotic stress. The results revealed 1003 DEGs, of which many associated with conidiation, xenotics transport, cell wall integrity, and protein and carbohydrate metabolism were greatly down-regulated. Total RNA obtained from Bbssk1 disruption mutant subjected to 0.5 M NaCl for 30 minutes compared to the wild type strain under the same stress treatment.