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Genome-wide Expression Profiles of Drought and High-salinity Stresses-responsive Genes in Rice (Oryza Sativa L. Indica)

ABSTRACT: Three rice major tissues, namely flag leaf, shoot and panicle, were involved in this study. Each tissue had two kinds stress treatment, drought and high salinity, in 3 different time courses. For drought treated samples, an additional water recovery was applied. Each experiment had three replicates. Keywords: Comparison of gene expression in three tissues with stress treatment and without treatment

ORGANISM(S): Oryza sativa

PROVIDER: GSE6533 | GEO | 2006/12/19

SECONDARY ACCESSION(S): PRJNA98809

REPOSITORIES: GEO

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Genome-wide Expression Profiles of Drought and High-salinity Stresses-responsive Genes in Rice (Oryza Sativa L. Indica)

Project description:Three rice major tissues, namely flag leaf, shoot and panicle, were involved in this study. Each tissue had two kinds stress treatment, drought and high salinity, in 3 different time courses. For drought treated samples, an additional water recovery was applied. Each experiment had three replicates. Keywords: Comparison of gene expression in three tissues with stress treatment and without treatment To globally elucidate potential genes involved in drought and high-salinity stresses responses in rice, an oligomer microarray covering 37,132 genes including cDNA or EST supported and putative genes was applied to study the expression profiling of shoot, flag leaf, and panicle under drought or high-salinity treatment. Three rice major tissues, namely flag leaf, shoot and panicle, were involved in this study. Each tissue had two kinds stress treatment, drought and high salinity, in 3 different time courses. For drought treated samples, an additional water recovery was applied. Each experiment had three replicates.

2010-06-05 | E-GEOD-6533 | biostudies-arrayexpress

Comparative transcriptomics and co-expression networks reveal tissue- and genotype-specific responses to reproductive-stage drought stress in rice (Oryza sativa L.) [panicle]

Project description:Purpose: The goal of the study is to compare the transcriptomic differences of the panicle tissue at the reproductive-stage of the Introgression Line (IR 96321-1447-165-B-3-1-2), which is drought-tolerant, to Swarna (recipient parent), which is drought-sensitive.

2020-02-25 | GSE145869 | GEO

Polysome bound mRNA Seq analysis of tolerant (APO) and susceptible (IR64) variety of Oryza sativa cv. Indica under 50% drought

Project description:Plants evolved several acquired tolerance traits for drought stress adaptation to maintain the cellular homeostasis. The combination of constitutive and acquired traits governs drought tolerance, which is crucial for maintaining crop productivity under drought. Drought affects protein synthesis, to uncover the translational landscape with response to drought stress in rice, polysome bound mRNA sequencing at anthesis stage in resistant APO and sensitive IR64 genotypes were performed. Our results demonstrate that drought tolerant genotype maintains higher transcripts bound to poly-ribosomes which facilitate higher protien synthesis which impacted on photosynthesis, spikelet fertility, seed filing and yield under drought stress. We identified many novel LncRNAs and relevant genes associated with translation which can play important role in manitaing grain protein content with drought tolerance.

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Oryza sativa Indica Group

Project description:rice young panicle Raw sequence reads

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Oryza sativa

Project description:The drought-responsive gene expression in rice panicle

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Small RNAs from 4-week old seedling of Oryza sativa

Project description:In order to identify new miRNAs, NAT-siRNAs and possibly abiotic-stress regulated small RNAs in rice, three small RNA libraries were constructed from control rice seedlings and seedlings exposed to drought or salt stress, and then subjected to pyrosequencing.

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Oryza sativa Indica Group

Project description:bulked RILs with high and low grain number per panicle derived from 2 cultivars at panicle primordia stage

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RNA Seq analysis of tolerant (BPT5204) and susceptible (TN1) variety of Oryza sativa cv. Indica under drought, pathogen, and combined stress

Project description:Climate change is affecting the unprecedented drought scenario and frequent occurrence of pathogen infection in rice. Simultaneous occurrence of these stresses could lead to more crop loss. Transcription response of genes involved in combined stress would provide relevant candidate gene to develop climate resilient rice. We report individual drought, Xoo infection and combined stress on rice. RNA Seq of contrasting genotypes BPT5204 and TN1 has revealed many candidate genes. A meta-analysis further filtered out some candidate genes which could be used for breeding programme. Several genes identified were already characterized by other groups for individual stress condition. However the genes involved in drought and pathogen infection could be further used for genetic manipulation studies in future.

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Oryza sativa cultivar:Cottondora sannalu

Project description:comparative rice panicle transcriptome under drought stress

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RNA-seq analyses of most resistance Indica Rice varieties (Oryza sativa L.) leaves tissue under water-deficit condition

Project description:In order to evaluate the genome differences and find the more tolerant cultivar, first eleven Malaysian rice cultivars namely, MR219, MR276, MR220, MR211, MR219-4, MR253, Q50, Q74, MR159, Masuri and MR263 were subjected under water deficit. Then, based on the morphological and physiological traits, the more drought-tolerant and -susceptible cultivars were screened and time-course gene expression profiling established by a comprehensive transcriptome database sequencing of the leaf RNA of tolerant rice. The current investigation provides pivotal data for understanding the rice drought tolerance mechanisms.

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