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Genome-wide map of SET1A occupancy in mouse ES cells

ABSTRACT: To identify SET1A genome-wide occupancy and further unveil its role in transcriptional regulation in mouse ES cells, we carried out chromatin immunoprecipitation followed by high sequencing (ChIP-seq).We established a stable ES cell line expressing 2X Flag tagged SET1A and performed ChIP with anti-Flag M2 beads, followed by deep sequencing. We found that the SET1A peaks show an outstanding enrichment in promoter region. Importantly, these SET1A binding loci revealed a clear co-localization with OCT4, and high H3K4me3 level, which is consistent with its interaction with OCT4 and intrinsic H3K4 methylase activity.

ORGANISM(S): Mus musculus

PROVIDER: GSE66067 | GEO | 2016/01/20

SECONDARY ACCESSION(S): PRJNA275806

REPOSITORIES: GEO

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