Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression in rat hippocampal CA1 during the consolidation of contextual fear memory

ABSTRACT: Adult rats (male Lister hooded) received a single unsignaled scrambled footshock (0.5 mA for 2 s) 2 min into a 3min exposure to a novel context. One half rats received bilateral microinfusions (1 microlitre/hemisphere) of 2 nmol/microlitre BDNF antisense ODN (BDNFASO, 5’-TCTTCCCCTTTTAATGGT-3’ in PBS, pH 7.4) or BDNF missense ODN (BDNFMSO, 5’ -ATACTTTCTGTTCTTGCC-3’ in PBS, pH 7.4) into the dorsal hippocampus (AP -3.50, relative to bregma), 90 min before contextual fear conditioning (CFC). Infusions were made in awake rats via previously surgically implanted indwelling stainless steel cannula. Rats were housed in the home cages at all times outside the conditioning procedure. Rats were killed by CO2 inhalation 2 hours after CFC. CA1 enriched dorsal hippocampus was immediately dissected on ice before rapid freezing and storage at -80oC. The BDNFASO-infused group represent consolidation-impaired rats.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE66150 | GEO | 2015/09/25

SECONDARY ACCESSION(S): PRJNA275973

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Gene expression in rat hippocampal CA1 after the retrieval of contextual fear memory in the absence of extinction.

Project description:Adult rats (male Lister hooded) received a single unsignaled scrambled footshock (0.5 mA for 2 s) 2 min into a 3min exposure to a novel context. 48 hours later, rats were reexopsed to the condition context for 2 min (TEST). One half rats received bilateral microinfusions (1 microlitre/hemisphere) of 2 nmol/microlitre Zif268 antisense ODN (ZIFASO, 5’- GGTAGTTGTCCATGGTGG-3’in PBS, pH 7.4) or Zif268 missense ODN (ZIFMSO, 5’ -GTGTTCGGTAGGGTGTCA-3’in PBS, pH 7.4) into the dorsal hippocampus (AP -3.50, relative to bregma), 90 min before TEST. Infusions were made in awake rats via previously surgically implanted indwelling stainless steel cannula. Rats were housed in the home cages at all times outside the conditioning and TEST procedures. Rats were killed by CO2 inhalation 2 hours after TEST. CA1-enriched dorsal hippocampus was immediately dissected on ice before rapid freezing and storage at -80oC. The ZIFSO-infused group potentially represents reconsolidation-impaired rats.

2015-09-25 | GSE66152 | GEO

Gene expression in rat hippocampal CA1 during the extinction of contextual fear memory

Project description:Adult rats (male Lister hooded) were pre-exposed for 3 d for 10 min /d (24 hr interval) to two different novel contexts. On day 4 rats received a single unsignaled scrambled footshock (0.5 mA for 2 s) 2 min into a 3min exposure to one of the two contexts. Rats were either returned to the CS conditioned context (CS+, EXT group) or to the other (CS-, NOR group) context for 10 min, 48h after contextual fear conditioning. Rats were housed in the home cages at all times outside the conditioning and re-exposure components. The novel context chosen to be the conditioned context was counterbalanced across the experimental groups. Rats were killed by CO2 inhalation 2 hours after CS- or CS+ exposure. CA1-enriched dorsal hippocampus was immediately dissected on ice before rapid freezing and storage at -80oC. The CS+ group represents rats that underwent extinction of contextual fear memory.

2015-09-25 | GSE66151 | GEO

Identification of HP0244 regulon at different pH conditions

Project description:Transcriptional profiling of Helicobacter pylori comparing 26695 wild-type strain and a HP0244-deficient mutant 26695/∆HP0244::km treated at three different pH conditions (pH 7.4, pH 4.5 without urea, or pH 2.5 with 30 mM urea) for 30 min to define the HP0244 acid-responsive regulon Keywords: Genetic modification and stress response

2009-09-14 | GSE12900 | GEO

Systems biology analysis of Korean red ginseng effects on Immobilization stressed mice brain

Project description:Here we present molecular mechanisms of Korean red ginseng (KRG) on immobilization stresses Keywords: stress response Mice were divided into three groups (3 mice / group): control, stress + no treat, and stress + Korean Red Ginseng (KRG, 100 mg). Stress + KRG group were given KRG 100 mg orally for 7 days and then exposed to immobilization stress for 45 min. stress + no treat group were administrated with phosphate buffer saline (d-PBS, pH 7.4) together with IMO stress for 45 min.

2010-05-15 | E-GEOD-12656 | biostudies-arrayexpress

K63 polyubiquitin targets in the hippocampus of male and female rats following contextual fear conditioning

Project description:This experiment examined K63 polyubiquitin protein targets in the male and female hippocampus after contextual fear conditioning. Samples were the CA1 region of the dorsal hippocampus collected from male and female Sprague-Dawley rats that were 8-9 weeks old. Samples were collected 1 hour after contextual fear conditioning (Trained) or from non-stimulated controls (Naïve).

2024-12-02 | PXD054726 | Pride

K63 polyubiquitin targets in the hippocampus of male and female rats following contextual fear conditioning

2024-12-02 | PXD054877 | Pride

Cell types in the mouse amygdala and their transcriptional response to fear conditioning

Project description:We performed single-cell RNA-seq of full, microdissected and dissociated mouse amygdala, 2h, 8h or 24h after tone-cued fear conditioning (CFC), and 2h after recall (exposure to CS only, 24h post-CFC), and naive homecage controls.

2023-01-01 | E-MTAB-12096 | biostudies-arrayexpress

Analysis of the effect of vascular endothelial growth factor on gene expression profile after focal cerebral ischemia in mouse

Project description:To investigate the effect of vascular endothelial growth factor (VEGF) on gene expression profile after focal cerebral ischemia in mouse, we employed Agilent SurePrint G3 Mouse Gene Expression 8×60K Microarray as a platform to identify which genes influenced by VEGF in mouse after focal cerebral ischemia. Mice were randomized to sham group, in which mice underwent sham surgery; MCAO group, in which transient (90 min) middle cerebral artery occlusion (MCAO) model was performed and mice received vehicle (PBS, 0.01M, pH 7.4) intracerebroventricularly in the right lateral ventricle 3hr after reperfusion; VEGF group (n = 36), in which rhVEGF-A165 (10μg/ml, dissolved in 0.01M PBS) was injected into the right lateral ventricle 3hr after reperfusion. Mice were sacrificed at 24hr after reperfusion, brains removed and peri-infarct areas were used for microarray. Gene expression microarray was applied to investigate the differentially expressed genes among sham group, MCAO group and VEGF group. Expression of thirty-seven genes was confirmed in the same RNA samples by real-time PCR.

2014-06-23 | GSE58720 | GEO

cow MFGM LC-MS/MS - Evaluation of six sample preparation procedures for qualitative and quantitative proteomics analysis of milk fat globule membrane

Project description:Pasteurized whole cow milk (Grade A) was purchased from the supermarket. Whole cow milk was centrifuged at 3 000 g at 4°C for 30 min and the fat layer was recovered. The recovered fat was washed with PBS for 20 min at 37°C, and the resuspened mixture was centrifuged again to obtain the milk fat. The wash procedure was repeated three times. Finally, milk fat was washed with MilliQ water to recover the fat globules. Milk fat globules were solubilized with a SDS solution (100 mM Tris-HCl, pH 7.4, 100 mM DTT and 4% SDS) and then incubated in 95°C water bath for 10 min. The mixtures were centrifuged at 14 000 g for 15 min, and the protein samples were collected. S-Trap, FASP, in-gel, and traditional Chl/MeOH, as well as modified chloroform/methanol (Chl/MeOH) or acetone precipitation followed by in-solution digestion without clean-up of tryptic peptides methods were applied to characterization cow MFGM proteome.

2018-10-19 | PXD009288 | Pride