Dataset Information

K63 polyubiquitin targets in the hippocampus of male and female rats following contextual fear conditioning

ABSTRACT: This experiment examined K63 polyubiquitin protein targets in the male and female hippocampus after contextual fear conditioning. Samples were the CA1 region of the dorsal hippocampus collected from male and female Sprague-Dawley rats that were 8-9 weeks old. Samples were collected 1 hour after contextual fear conditioning (Trained) or from non-stimulated controls (Naïve).

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain

SUBMITTER: Keith Ray

LAB HEAD: Dr. Timothy J. Jarome

PROVIDER: PXD054726 | Pride | 2024-12-02

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	240417_Natalie_M1_01.raw	Raw
	240417_Natalie_M1_02.msf	Msf
	240417_Natalie_M1_02.msfView	Msf
	240417_Natalie_M1_02.mzML	Mzml
	240417_Natalie_M1_02.mzid.gz	Mzid

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Publications

Decreases in K63 Polyubiquitination in the Hippocampus Promote the Formation of Contextual Fear Memories in Both Males and Females.

Preveza Natalie J NJ Setenet Gueladouan G Gwin Phillip P Bae Yeeun Y Patrick Morgan B MB Cummings Adam A Abraham Jennifer R JR Ray W Keith WK Helm Richard F RF Jarome Timothy J TJ

Hippocampus 20250101 1

Over 90% of protein degradation in eukaryotic cells occurs through the ubiquitin-proteasome system (UPS). In this system, the ubiquitin protein can bind to a substrate on its own or it can form a chain with multiple ubiquitin molecules in a process called polyubiquitination. There are 8 different sites on ubiquitin at which polyubiquitin chains can be formed, the second most abundant of which, lysine-63 (K63), is independent of the degradation process, though this mark has rarely been studied in ...[more]

PMID: 39610227

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