Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Role of beclin-1 in breast cancer microenvironment

ABSTRACT: A comprehensive gene expression analysis was performed on a co-culture of breast cancer cells and mesenchymal stromal cells, the latter of which either expressed beclin-1 or was depleted of beclin-1 by siRNA.

ORGANISM(S): Homo sapiens

PROVIDER: GSE66154 | GEO | 2015/02/21

SECONDARY ACCESSION(S): PRJNA275976

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Beclin-1 is dispensable for chromosome congression and proper outer kinetochore assembly

Project description:Chromosomal instability (CIN) is defined by the propensity to acquire structural and/or numerical aberration in the normal cellular karyotype and is often associated with cancer. Autophagy is a catabolic process that leads to the recycling of cellular components that may positively or negatively impact on cancer development and progression, depending on the context. Recent work postulated that the depletion of the pro-autophagic and tumor suppressive protein Beclin 1 triggers CIN by interfering with mitotic chromosome segregation, providing a possible mechanism for how Beclin 1 can act as a tumor suppressor (Fremont et al., PMID: 23478334). Here, we present data supporting the notion that the phenotypes described in Fremont et al., depend on a siRNA off-target effect. The transcriptomic analysis shown here was designed to identify the factor(s) that are responsible for such phenotype. HeLa cells synchronized in mitosis have been transfected in 3 independent experiments with either Beclin 1-ER or Beclin 1-II siRNA. RNA was extracted and subjected to gene expression profiling. Differentially expressed probe sets/genes have been identified between the ER and II siRNA using the moderated t-test from Bioconductor's limma package with the resulting p-values being adjusted for multiple hypothesis testing using the method from Benjamini and Hochberg.

2015-09-02 | E-GEOD-69052 | biostudies-arrayexpress

Beclin-1 is dispensable for chromosome congression and proper outer kinetochore assembly

2015-09-02 | GSE69052 | GEO

Depletion of ADAM12+ MSCs normalizes the tumor stromal microenvironment

Project description:This study investigates transcriptomic changes occurring in PDPN+PDGFRa+ stromal cells from murine B16-OVA melanomas (MO5) when ADAM12+ mesenchymal stromal cells (MSCs) are depleted.

2023-10-05 | GSE206794 | GEO

RNAseq analysis of tissue and primary cell transcriptomes from wild type and Beclin 2 KO mice.

Project description:Our study represents the first detailed analysis of WT vs Beclin 2 KO cell/tissue transcriptomes, with biologic replicates, generated by RNA-seq technology. RNA-seq based transcriptome characterization would expedite genetic network analyses and permit the dissection of complex biologic functions.

2021-03-06 | GSE111539 | GEO

Extracellular matrices of stromal cell subtypes regulate phenotype and contribute to the stromal microenvironment in vivo

Project description:Comparison of secreted proteins from heterogeneous mesenchymal stromal cells.

2024-05-29 | MSV000094888 | MassIVE

Diabetic microenvironment deteriorates the regenerative capacities of adipose mesenchymal stromal cells

Project description:Human adipose mesenchymal stromal cells cutltured in culture media containing diabetic patient serum or normal indvidual serum as control for 6 days and 1 month period

2024-06-22 | PXD036116 | Pride

Pivotal role of HMGA1 gene signature in highly metastatic breast cancer

2013-12-10 | GSE35525 | GEO

Pivotal role of HMGA1 gene signature in highly metastatic breast cancer

Project description:Analysis of MDA-MB-231 breast cancer cells depleted for High Mobility Group A1 (HMGA1) using siRNA. HMGA1 is involved in invasion and metastasis in breast cancer cells. Results identify the specific transcriptional program induced by HMGA1 in highly metastatic breast cancer cells. MDA-MB-231 cells were transfected with HMGA1-specific siRNA or a control siRNA. Transfections were performed by using Lipofectamin RNAiMAX (Invitrogen) according to the manufacturer's procedure. Seventy-two hours after transfection, samples were processed for total RNA extraction and hybridization on Affymetrix microarrays. Four biological replicas (A, B, C, D) were used for each of the two conditions, for a total of 8 samples.

2013-12-10 | E-GEOD-35525 | biostudies-arrayexpress

Genome-wide analysis of miRNA-mRNA interactions in the marrow microenvironment (HTS)

Project description:We report genome-wide analysis of miRNA-mRNA interactions in the hematopoietic marrow microenvironment (ME) by employing the biochemical technique. High-throughput sequencing of RNA isolated by crosslinking immunoprecipitation or HITS-CLIP. Specifically, we analyzed 3 kinds of stromal cells (two human stromal cell lines named HS5 and HS27a and primary mesenchymal stromal cells (MSC) from normal donors) and two types of endothelial cells (TrBMEC, a human bone marrow endothelial cell line) and HUVEC (Human Umbilical Vein Endothelial Cells). Immune precipitation (IP) of Argonaut proteins (AGO1-4) was performed by anti-AGO monoclonal antibody 2A8. IP was performed on cells cross-linked by short-wave ultraviolet radiation (UV) 365 nM. Protocol was modified from as originally published by Chi et al Nature. 2009 Jul 23;460(7254):479-86.

2013-09-01 | E-GEOD-41272 | biostudies-arrayexpress

Role of Eif4g3 and Deaf1 in Lymph node

Project description:This SuperSeries is composed of the following subset Series: GSE29152: Lymph node stromal cells: Control siRNA treated vs. Eif4g3 siRNA treated GSE29153: Differential gene expression in the Pancreatic lymph node of Deaf1 knockout mice vs. wild type littermate controls Refer to individual Series

2011-08-31 | E-GEOD-29167 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data