Frequent derepression of the mesenchymal transcription factor gene in acute myeloid leukemia (human)
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ABSTRACT: Bone marrow samples from normal adult male donors were collected into EDTA. Red cells were removed by ammonium chloride lysis. Leukocytes were washed in SM buffer and CD34+ cells were separated from CD34- cells using an AutoMACS device and anti-CD34 immunomagnetic beads (Miltenyi Biotec), according to manufacturer’s instructions. For mature cell populations, CD34- cells were FACS purified according to the following immunophenotypes, with 7-AAD used to exclude dead cells: Neutrophils: side scatter high CD15+ CD16+. Monocytes: side scatter low-intermediate CD14+ CD16- CD15-. See also Huang et al., 2014.
ORGANISM(S): Homo sapiens
PROVIDER: GSE66254 | GEO | 2015/09/17
SECONDARY ACCESSION(S): PRJNA276269
REPOSITORIES: GEO
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