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Analysis of WRKY33 binding sites and WRKY33-dependent gene expression in Arabidopsis thaliana upon Botrytis cinerea 2100 inoculation

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE66300 | GEO | 2015/06/15

SECONDARY ACCESSION(S): PRJNA276454

REPOSITORIES: GEO

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WRKY33 binding sites in Arabidopsis upon Botrytis cinerea 2100 inoculation

Project description:The Arabidopsis thaliana mutant wrky33 is highly susceptible to the necrotrophic fungus Botrytis cinerea. We identified by ChIP-seq >1680 high-confidence WRKY33 binding sites associated with 1576 genes within the Arabidopsis genome, with all of them being dependent on rapid activation of WRKY33 expression by Botrytis cinerea strain 2100. Genome-wide transcriptional analysis defined 318 genes as direct functional targets at 14 h post inoculation. Comparison between resistant wild-type Columbia-0 and susceptible wrky33 mutant plants revealed that expression of 75% of all WRKY33 regulated targets were down-regulated upon infection, indicating that WRKY33 predominately acts as a repressor. However, WRKY33 appears to possess dual functionality acting either as a repressor or as an activator in a promoter-context dependent manner. Our genome-wide analysis confirmed known WRKY33 targets involved in ethylene and jasmonic acid hormone signaling and phytoalexin biosynthesis, but also uncovered a previously unknown role of abscisic acid (ABA) biosynthesis in the complex regulatory circuitry affecting resistance towards Botrytis.

2015-06-15 | GSE66289 | GEO

WRKY33 binding sites in Arabidopsis upon Botrytis cinerea 2100 inoculation

2015-06-15 | E-GEOD-66289 | biostudies-arrayexpress

Analysis of WRKY33 binding sites and WRKY33-dependent gene expression in Arabidopsis thaliana upon Botrytis cinerea 2100 inoculation

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-06-15 | E-GEOD-66300 | biostudies-arrayexpress

WRKY33-dependent expression of Arabidopsis genes upon Botrytis cinerea 2100 inoculation

Project description:The Arabidopsis thaliana mutant wrky33 is highly susceptible to the necrotrophic fungus Botrytis cinerea. Comparing the expression profiles of B. cinerea-infected wrky33 and WT plants we identified 2765 differentially expressed genes dependent on WRKY33, of which 1675 were up-regulated in the mutant (termed WRKY33-repressed genes) and 1090 were down-regulated in the mutant. Combined with ChIP-seq data 318 genes were identified as direct functional targets of WRKY33 at 14 h post inoculation with spores of Botrytis cinerea 2100.

2015-06-15 | GSE66290 | GEO

WRKY33-dependent expression of Arabidopsis genes upon Botrytis cinerea 2100 inoculation

2015-06-15 | E-GEOD-66290 | biostudies-arrayexpress