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Gene expression in undifferentiated or cyclic adenosine monophosphate-exposed BeWo trophoblast cells

ABSTRACT: BeWo trophoblast cells differentiate in response to expsure to cyclic adenosine monophosphate (cAMP) analogs. Differentiation includes syncytialization (fusion) and hormonogenesis. The goal of this study was to globally determine transcripts differentially expressed in BeWo trophoblast cells following a 24-h exposure to 250 uM 8-bromo-cAMP.

ORGANISM(S): Homo sapiens

PROVIDER: GSE66840 | GEO | 2015/08/01

SECONDARY ACCESSION(S): PRJNA278069

REPOSITORIES: GEO

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Gene expression in undifferentiated or cyclic adenosine monophosphate-exposed BeWo trophoblast cells

Project description:BeWo trophoblast cells differentiate in response to expsure to cyclic adenosine monophosphate (cAMP) analogs. Differentiation includes syncytialization (fusion) and hormonogenesis. The goal of this study was to globally determine transcripts differentially expressed in BeWo trophoblast cells following a 24-h exposure to 250 uM 8-bromo-cAMP. 3 replicates undifferentiated BeWo trophoblast cells; and 3 replicates BeWo trophoblast cells treated with 250 uM 8-Br-cAMP for 24 h.

2015-08-01 | E-GEOD-66840 | biostudies-arrayexpress

Effect of OVO-like 1 knockdown on global transcript expression in differentiated BeWo trophoblast cells

Project description:We had previously discovered that the transcription factor OVO-like 1 (OVOL1) was highly induced during trophoblast differentiation. In this study, we used an lentiviral shRNA strategy to decrease OVOL1 expression in BeWo trophoblast cells. Control cells were transduced with shRNAs targeting no known mammalian transcript (shCont). Following stimulation of differentiation (48h exposure to 8-bromo-cyclic adenosine monophosphate), a RNA-seq approach was used to determine global transcript differences in OVOL1-knockdown cells compared to control cells. Trophoblast cells transduced with control shRNAs were used as controls. Cells transduced with shRNAs targeting OVOL1 were used as treatment. All cells received 250 uM 8-bromo-cyclic adenosine monophosphate to stimulate differentiation. Three independent replicates of control and treatment groups were analyzed.

2015-08-01 | E-GEOD-66962 | biostudies-arrayexpress

Effect of OVO-like 1 knockdown on global transcript expression in differentiated BeWo trophoblast cells

2015-08-01 | GSE66962 | GEO

Expression data from BeWo cells treated without or with PKA- or EPAC-selected cAMP analogs

Project description:Cyclic AMP activates two downstream factors, protein kinase A (PKA) and exchange protein directly activated by cAMP (EPAC) and both downstream signalings induce syncytialization, cell fusion and the production of hCG and progesterone. We used microarray to identify novel transcription factors related to syncytialization in two cAMP signaling-stimulated BeWo cells.

2017-09-30 | GSE100095 | GEO

Transcriptomic Profiling of Trophoblast Fusion Using BeWo and JEG-3 Cell Lines

Project description:Introduction: In human placenta, alteration in trophoblast differentiation has a major impact on placental maintenance and integrity. Moreover, abnormal syncytial fusion seems to be implicated in the development of many complications including pre-eclampsia and intra-uterine growth restriction (IUGR). However, little is known about the mechanisms that control cytotrophoblast fusion into syncytiotrophoblast. BeWo cell line is commonly used to study placental function since it can be induced to form syncytium and secretes hormones. This fusion is triggered by cAMP or forskolin treatments. In contrast, JEG-3 cell line fails to undergo substantial fusion. Therefore, we will use BeWo and JEG-3 cells to identify a set of genes responsible of trophoblast fusion. Methods: BeWo and JEG-3 cells were treated with forskolin for 48 to induce fusion. RNA was extracted, hybridized to Affymetrix HuGene ST1.0 arrays and analyzed using system biology. Trophoblast differentiation was evaluated by real time PCR and immunocytochemistry analysis. Moreover, some of the identified genes were validated by real time PCR. Results: Our results identified a list of 32 altered genes in fused BeWo cells compared to JEG-3 cells after forskolin treatment. Among these genes, 4 were validated by real-time PCR including salt inducible Kinase 1 (SIK1) gene which is specifically upregulated in BeWo cells upon fusion. Moreover, using system biology analysis, SIK1 showed to be at the center of many biological and functional processes which suggest that it might play a major role in trophoblast differentiation. Conclusion: This study identified new target genes implicated in trophoblast fusion; a process implicated in the etiology of many pregnancy-related diseases such as preeclampsia, IUGR, and gestational diabetes.

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Biphasic response of Protein Kinase A to cyclic adenosine monophosphate triggers distinct epithelial phenotypes

Project description:We postulated that the ability of bPAC-generated cAMP to induce the biphasic response of PKA might have profound and distinct effects on the cellular transcriptome. To test this hypothesis, we carried out mRNA sequencing of MDCKI and MDCKI+bPAC cells under no, low, or high cAMP inputs (0%, 1.7% and 33% blue light duty cycle)

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Project description:Analysis of the effect of ZNF554 knock-down on genome-wide gene expression in BeWo trophoblast-like cells. The hypothesis tested in the present study was that ZNF554 regulates the expression of genes involved in key functions of trophoblastic cells. The results provide important information on the functions of ZNF554 in BeWo trophoblast-like cells.

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Forskolin induction of fusion in BeWo trophoblast cells

Project description:Trophoblast fusion is a central step in placental physiology. This mechanisms can be studied in the BeWO cell model that fuse following forskolin treatment that activates the c-AMP cascade.

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Protein interactome of 3’,5’-cyclic adenosine monophosphate reveals its role in regulating the actin cytoskeleton

Project description:Four biological replicates of Arabidopsis seedlings supplemented with 1 µM Br-3’,5’-cAMP, or water (control) were subjected to untargeted proteomics analysis at two time-points: 30 min and 1 h. Rather than wild-type, we used act2act7 mutant because of the evident phenotypic changes associated with the 3’,5’-cAMP supplementation.

2023-05-22 | PXD030124 | Pride

Expression data for the siCtrl and siHPIP treated BeWo cells

Project description:HPIP is an oncogenic protein and plays a major role in cancer progression, while its role in trophoblast differentiation is largly unknown. Therefore we analysed the effect of HPIP knockdown on gene expression signature in BeWO cells.

2021-03-21 | GSE159953 | GEO

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