Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Small RNAs during E. histolytica stress response

ABSTRACT: We analyzed ~27nt small RNAs from Entamoeba histolytica trophozoites in basal conditions and after heat shock or oxidative stress

ORGANISM(S): Entamoeba histolytica

PROVIDER: GSE67230 | GEO | 2015/07/22

SECONDARY ACCESSION(S): PRJNA279352

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Small RNAs during E. histolytica stress response

Project description:We analyzed ~27nt small RNAs from Entamoeba histolytica trophozoites in basal conditions and after heat shock or oxidative stress E. histolytica trophozoites were treated with 1mM H2O2 for 1hr, or heat shocked at 42°C for 1hr and RNA was isolated and small RNA populations were compared to small RNA populations from untreated trophozoites

2015-07-22 | E-GEOD-67230 | biostudies-arrayexpress

Comparison of normal and heat shock trophozoites

2007-03-01 | GSE6650 | GEO

Small RNAs during E. invadens stage conversion

Project description:We analyzed ~27nt small RNAs from Entamoeba invadens trophozoites, 24h cysts, 72h cysts, and excysting cells (8h)

2015-07-22 | GSE67231 | GEO

Comparison of normal and heat shock trophozoites

Project description:We examined the stress response in Entamoeba histolytica trophozoites by comparing untreated log-phase HM-1:IMSS trophozoites to those subjected to heat shock at 42C for 1 hour. Keywords: stress reponse We compared two arrays from normal trophozoites to two arrays from trophozoites subjected to heat shock.

2007-03-01 | E-GEOD-6650 | biostudies-arrayexpress

Gene expression in phagocytic and non-phagocytic Entamoeba histolytica populations

2012-10-16 | GSE41604 | GEO

Gene expression in phagocytic and non-phagocytic Entamoeba histolytica populations

Project description:The ability of Entamoeba histolytica to phagocytose host cells correlates to observed virulence in vivo. To better understand the mechanism of phagocytosis we used paramagnetic beads coated with host ligand and sorted trophozoites based on phagocytic ability. Gene expression was then measured in both the sorted phagocytic and non-phagocytic populations using a custom Affymetrix chip for E. histolytica. Feed forward regulation of phagocytosis by Entamoeba histolytica. Infection and Immunity. PMID 23045476 M280 Streptavidin Dynabeads (Invitrogen) were labeled with 20ug/mL biotinylated Human C1q (Quidel). Entamoeba histolytica (strain HM1) were washed twice with PBS then resuspended with the labeled beads at a 10:1 ratio of beads to trophozoites. Samples were incubated at 37Â°C for 45 minutes, washed twice with agitation to remove adherent beads, then resuspended in MACS buffer. Samples were loaded into magnetic columns (Miltenyi Biotec) and trophozoites were seperated according to manufacturer's protocols. phagocytic vs. non-phagocytic Entamoeba histolytica populations

2012-10-16 | E-GEOD-41604 | biostudies-arrayexpress

Comparative Proteomic Study of Entamoeba histolytica Trophozoites, Cysts, and Cyst-Like Structures

Project description:Proteomic comparison between trophozoites, cysts and cyst-like structures of Entamoeba histolytica

2016-04-21 | MSV000079674 | MassIVE

Small RNAs during E. invadens stage conversion

Project description:We analyzed ~27nt small RNAs from Entamoeba invadens trophozoites, 24h cysts, 72h cysts, and excysting cells (8h) E. invadens trophozoites were induced to encyst by incubation in low glucose media, and parasites harvested at 0, 24 and 72h. A subset of the 72h cysts were induced to excyst, and parasites harvested at 8h. Total RNA was extracted for each sampleand small RNA libraries constructed and sequenced as below

2015-07-22 | E-GEOD-67231 | biostudies-arrayexpress

Transcription profiling of Entamoeba histolytica trophozoites in mid-logarithmic growth

Project description:Entamoeba histolytica is a protozoan parasite which causes colitis and liver abscesses. Using a genomic DNA microarray consisting of 1.6 - 2.0 kb genomic inserts we have generated a transcriptional profile of 1,971 unique parasite transcripts. The 12 arrays in this experiment set were used to estimate relative transcript abundance for Entamoeba histolytica (HM-1:IMSS) trophozoites in mid-logarithmic growth.

2005-07-06 | E-SMDB-3205 | biostudies-arrayexpress

Entamoeba histolytica

Project description:Gene expression in Entamoeba histolytica cysts and trophozoites

| PRJNA98629 | ENA