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Mutational analysis of measles virus suggests constraints on antigenic variation of the glycoproteins

ABSTRACT: RNAseq of mutant measles virus libraries

ORGANISM(S): Measles morbillivirus

PROVIDER: GSE67666 | GEO | 2015/04/08

SECONDARY ACCESSION(S): PRJNA280602

REPOSITORIES: GEO

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Mutational analysis of measles virus suggests constraints on antigenic variation of the glycoproteins

Project description:RNAseq of mutant measles virus libraries Insertional mutagenesis of measles to identify tolerated locations Mutant measles virus libraries were constructed as described in: Heaton, N.S., Sachs, D., Chen,C.J., Hai, R., and Palese, P. (2013). Genome-wide mutagenesis of influenza virus reveals unique plasticity of the hemagglutinin and NS1 proteins. PNAS. 110, 20248-20253.

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Mutational Analysis of Measles Virus Suggests Constraints on Antigenic Variation of the Glycoproteins.

Project description:Measles virus undergoes error-prone replication like other RNA viruses, but over time, it has remained antigenically monotypic. The constraints on the virus that prevent the emergence of antigenic variants are unclear. As a first step in understanding this question, we subjected the measles virus genome to unbiased insertional mutagenesis, and viruses that could tolerate insertions were rescued. Only insertions in the nucleoprotein, phosphoprotein, matrix protein, as well as intergenic regions were easily recoverable. Insertions in the glycoproteins of measles virus were severely under-represented in our screen. Host immunity depends on developing neutralizing antibodies to the hemagglutinin and fusion glycoproteins; our analysis suggests that these proteins occupy very little evolutionary space and therefore have difficulty changing in the face of selective pressures. We propose that the inelasticity of these proteins prevents the sequence variation required to escape antibody neutralization in the host, allowing for long-lived immunity after infection with the virus.

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