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Gene expression profiling of B-cell and non-B-cell fractions of follicular lymphoma

ABSTRACT: Excisional biopsies of lymph nodes involved by follicular lymphoma (FL) were obtained from previously-untreated, advanced-stage patients to generate heterohybridomas and idiotype vaccine for a clinical trial (PMID: 21632504). Residual, viably-cryopreserved suspensions of disaggregated cells were magnetically sorted by negative selection into nominal B (CD3-depleted) and non-B (NB; CD19- and CD20-depleted) fractions. Total RNA was extracted from each fraction for separate gene expression profiling (GEP). Technically-satisfactory GEP results were obtained from both fractions for 43 patients.

ORGANISM(S): Homo sapiens

PROVIDER: GSE68092 | GEO | 2019/04/20

REPOSITORIES: GEO

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