Other

Dataset Information

0

Space environmental factor impacts upon murine colon microbiota and mucosal homeostasis


ABSTRACT: We report how high and low linear energy transfer (LET) radiation, microgravity, and elevated dietary iron affect colon microbiota (determined by 16S rDNA pyrosequencing) and colon function. Three independent experiments were conducted: 1) fractionated low LET γ radiation (137Cs, 3 Gy, RAD), high Fe diet (IRON) (650 mg/kg diet), and a combination of low LET γ radiation and high Fe diet (IRON+RAD) in male Sprague-Dawley rats; 2) high LET 38Si particle exposure (0.050 Gy), 1/6 G partial weight bearing (PWB), and a combination of high LET38Si particle exposure and PWB in female BalbC/ByJ mice; and 3) 13 d spaceflight in female C57BL/6 mice. For each experiment, the colon was resected and feces removed for microbial sequencing analysis on a Roche 454 Genome Sequencer FLX Titanium instrument (Microbiome Core Facility, Chapel Hill NC) using the GS FLX Titanium XLR70 sequencing reagents and protocols. Analysis of amplicon sequencing data was carried out using the QIIME pipeline. Low LET radiation, high iron diet, and spaceflight increased Bacteroidetes and decreased Firmicutes. Low LET radiation, high Fe diet, and spaceflight did not significantly affect diversity or richness, or elevate pathogenic genera. Spaceflight increased Clostridiales and decreased Lactobacillales, and similar trends were observed in the experiment using a ground-based model of microgravity, suggesting altered gravity may affect colonic microbiota. Microbiota characteriztion in these models is a first step in understanding the impact of the space environment on intestinal health.

ORGANISM(S): mouse gut metagenome rat gut metagenome

PROVIDER: GSE68738 | GEO | 2015/11/30

SECONDARY ACCESSION(S): PRJNA283802

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2023-08-11 | GSE230674 | GEO
| 2274754 | ecrin-mdr-crc
2021-09-30 | GSE184759 | GEO
2014-03-18 | GSE53254 | GEO
2013-06-05 | E-GEOD-42642 | biostudies-arrayexpress
2021-05-24 | GSE161646 | GEO
2021-05-24 | GSE161644 | GEO
2017-01-20 | GSE93838 | GEO
| PRJNA714661 | ENA
2013-06-29 | GSE48416 | GEO