Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Development of Regulatory T cell Precursors in the Absence of a Functional Foxp3 Protein

ABSTRACT: To analyze gene expression in in regulatory T cell precursors that develop in the absence of a functional Foxp3 protein as compared to that of normal regulatory T cells Keywords: Cell type comparison

ORGANISM(S): Mus musculus

PROVIDER: GSE6875 | GEO | 2007/01/26

SECONDARY ACCESSION(S): PRJNA99127

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcription profiling of mouse CD4+ T cells expressing Foxp3 FACS isolated from hemizygouse males to investigate development of regulatory t cell precursors in the absence of a functional foxp3 protein

Project description:To analyze gene expression in in regulatory T cell precursors that develop in the absence of a functional Foxp3 protein as compared to that of normal regulatory T cells Experiment Overall Design: Murine wild-type and mutant (delta) Foxp3 alleles were tagged with EGFP reporters. CD4 positive T cells expressing the respective tagged Foxp3 allele (Foxp3/EGFP and delta Foxp3/EGFP) were isolated from hemizygous male mice by cell sorting using FACS. CD4 positive cells that did not express the EGFP tag were a isolated. Total RNA was prepared and used in the array studies.

2008-06-15 | E-GEOD-6875 | biostudies-arrayexpress

Identify proteins at Foxp3 bound cis-regulatory elements in situ

Project description:We hypothesize that nuclear factors co-occupying the genetic elements with regulatory T (Treg) cell lineage–specifying factor Foxp3 play critical roles in transcriptional regulation of Treg immune suppression function, thus, offering a unique approach to investigate the factors and their mechanisms controlling Treg-mediated immune tolerance involved in self-tolerance and antitumor immunity. We seek to identify the proteins occupying Foxp3 targets in the resting state or after cells receiving stimulation. To this end, we projected the spatial information (PSI) of Foxp3, Histone H3, or Stat5 onto their adjacent proteins with peroxidase–catalyzed biotin-phenoxyl radicals and identify these biotinylated proteins with tandem mass tag (TMT)–based quantitative mass spectrometry (MS).

2024-06-08 | PXD019050 | Pride

scRNAseq transcriptome analysis of developing regulatory T cells and precursors in the thymus

Project description:We analyzed the individual transcriptomes of thymic Treg cells (CD4+Foxp3+), their immediate precursors (CD25+CD4+Foxp3-) and mature CD4 single positive thymocytes (CD4+Foxp3-CD25-CD62L+CD24-).

2019-08-19 | GSE134902 | GEO

Characterization of a human regulatory T cell-like cell line, MT-2, in the presence or absence of FOXP3

Project description:These studies profiled the transcriptional differences in a human regulatory T cell-like cell line, MT-2, when FOXP3 a master transcriptional regulatory element was present or absence. In the FOXP3 null MT-2 cell line FOXP3 express had been deleted by CRISPR-Cas9. RNA was prepared for bulk RNA-seq from 4 replicates for each cell line. In the absence of FOXP3 in the MT-2 cell line there were 1251 differential expressed genes. These differentially expressed gene results indicate that hte loss of FOXP3 in MT-2 cells results in altered transcripts and pathways often associated with regultory T cell activity or phenotypes.

2023-09-21 | GSE220629 | GEO

Foxp3 ablation in peripheral mature regulatory T cells

2007-01-25 | GSE6681 | GEO

Transcription profiling of mouse natural and induced regulatory T cells

Project description:The gene expression profile of peripheral Foxp3+ natural regulatory T cells isolated from Foxp3/EGFP bicistronic mice was compared to that of in vitro-induced regulatory T cells and to CD4+ conventional (Foxp3-) T cells. The role of the regulatory T cell transcription factor Foxp3 in shaping the transcriptosomes of natural and induced regulatory T cells was analyzed using mice expressing a mutant FOXP3-EGFP fusion protein (Foxp3deltaEGFP). We used gene expression microarrays to examine the transcriptional programs of natural and induced regulatory T cells and the function of Foxp3 in organizing the transcriptosomes of the respective cell type Experiment Overall Design: Conventional T cells and natural and induced regulatory T cells were derived from Foxp3/EGFP bicistronic mice and analyzed for their gene expression profile. Conventional T cells, regulatory T cell precursors (CD4+Foxp3deltaEGFP+) and induced regulatory T cell precursors (CD4+Foxp3deltaEGFP+) cells were deriv ed from Foxp3deltaEGFP mice

2009-03-07 | E-GEOD-14415 | biostudies-arrayexpress

Transcription profiling of mouse Foxp3 ablation in peripheral mature regulatory T cells

Project description:Analysis of Foxp3 ablated peripheral regulatory T cells. Regulatory T cells require the expression of the transcription factor Foxp3 for thymic development. It is not known whether continuous expression of Foxp3 is required for the maintained function of mature regulatory T cells in the periphery. Results indicate changes to the regulatory T cell developmental program in the absence of Foxp3. Experiment Overall Design: Compare Cre recombinase treated peripheral regulatory T cells possessing a Cre sensitive Foxp3 locus to Cre treated regulatory T cells with a wild type Foxp3 locus. Cre exposure is observed via the Cre sensitive expression of the yellow flourescent protein molecule.

2008-06-15 | E-GEOD-6681 | biostudies-arrayexpress

HIC1 interacts with FOXP3 multi protein complex: a novel mechanism to regulate human regulatory T cell differentiation and function

Project description:Recent data have shown that Hypermethylated in cancer 1 (HIC1) is an important contributor to iTreg cell development and function. Using affinity-purification and tandem mass spectrometry we systematically characterized the HIC-1 interactome in human iTreg cells. On the basis of these data, we have shown that HIC1 is a part of FOXP3-RUNX1-CBFβ protein complex that regulates Treg signature genes and is indispensable for the suppressive function of FOXP3+ regulatory T cells. SRM was used to validate HIC1 interactors

2023-10-16 | PXD039337 | Pride

Gene expression profile of Foxp3 expressing naturally ocurring regulatory macrophages from mice

Project description:Define the genetic profile of naturally occuring regulatory macrophages that express Foxp3 (MacRegs) compared to Foxp3 neg macrophages, to determine candidate genes responsible for their regulatory function. Compare this new cellular population with Tregulatory cells. Two conditions were compared: Fresh CD11b+ F4/80+ FOXP3+ cells (3 independent isolates) and CD11b+ F4/80+ FOXP3- cells (3 independent isolates). MacRegs were also compared to CD4+Foxp3+ Tregs .

2011-04-01 | E-GEOD-23793 | biostudies-arrayexpress

Regulatory T cell lineage specification by Foxp3

Project description:Examination of CD4+ T cells from Foxp3-GFP knock-in mice. Aim is to understand the genetic program governed by Foxp3 in T cells by comparison of CD4 T cells subdivided into four groups based on expression of Foxp3 and CD25. Keywords = regulatory T cells Keywords = Foxp3 Keywords: repeat sample

2005-03-23 | GSE2389 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data