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In vitro model of tumor-associated macrophages

ABSTRACT: The tumor microenvironment is highly complex and consists af many non-tumor cells and factors. To simplify the complexity, we developed a simple in vitro system where tumor (neuroblastoma) 'spheres' ~1-2 mm in diameter were generated on concave surfaces of noble agar. After sphere development, bone marrow-derived macrophages were added to the well. Many macrophages crawled into the spheres (as shown by live cell microscopy). After 24-28 h, the macrophages were isolated from inside or outside the tumor spheres using tissue digestion and anti-CD11b MACs beads. The macrophages from inside or outside the spheres were then subject to microarray analysis.

ORGANISM(S): Mus musculus

PROVIDER: GSE68817 | GEO | 2016/05/02

SECONDARY ACCESSION(S): PRJNA283824

REPOSITORIES: GEO

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