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Overexpression of a panel of miRNA mimics in DU145 prostate cancer cells.

ABSTRACT: The role of a number of miRNAs found to be differentially expressed in prostate cancer was functionally investigated by overexpressing them in DU145 prostate cancer cells and by analyzing gene expression profiles.

ORGANISM(S): Homo sapiens

PROVIDER: GSE68883 | GEO | 2017/06/01

SECONDARY ACCESSION(S): PRJNA283973

REPOSITORIES: GEO

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Controls and CNTN1 overexpression in DU145 cells and CNTN1 knockdown in DU145 cell-derived prostate cancer stem-like cells

Project description:Prostate cancer stem-like cells were derived from DU145 cells as suspension spheres. DU145 cells were transduced with a CNTN1 over expressing retroviral vector and a control empty vector. DU145 spheres were transduced using a retroviral-based shRNA vector against CNTN1 and a scrambled control shRNA viral vector. Both cell lines were cultured over a couple of passages before RNA was collected.

2016-02-12 | E-MTAB-4118 | biostudies-arrayexpress

Modulation of ST6Gal1 in Prostate Cancer Cells

Project description:Proteomics analyses using LC-MS/MS was performed on wild-type and ST6Gal1 overexpressing DU145 prostate cancer cells. Total cell lysate and secreted proteins from both were analyzed.

2023-11-06 | PXD041316 | Pride

Study of matrix proteins synthesized by DU145 and PC3 prostate cancer cells alone or in co-culture with prostate-derived fibroblasts.

Project description:Tumor microenvironment or stroma has the potency to regulate the behavior of malignant cells. Fibroblast-like cells are abundant in tumor stroma and they are also responsible for the synthesis of many extracellular matrix components. Fibroblast–cancer cell interplay can modify the functions of both cell types. We applied mass spectrometry and proteomics to unveil the matrisome in 3D spheroids formed by DU145 prostate cancer cells, PC3 prostate cancer cells or prostate derived fibroblasts. Similarly DU145/fibroblast and PC3/fibroblast co-culture spheroids were also analyzed. Western Blotting and immunofluorescence were used to confirm the presence of specific proteins in spheroids. Cancer dissemination was studied by utilizing "out of spheroids" migration and invasion assays. In the spheroid model cancer cell–fibroblast interplay caused remarkable changes in extracellular matrix and accelerated the invasion of DU145 cells. Fibroblasts produced structural matrix proteins, growth factors and matrix metalloproteinases. In cancer cell/fibroblast co-cultures basement membrane components, including laminins (3, 5, 2, 3), heparan sulphate proteoglycan (HSPG2 gene product), and collagen XVIII accumulated in a prominent manner when compared to spheroids that contained fibroblasts or cancer cells only. Furthermore, collagen XVIII was intensively processed to different endostatin isoforms by cancer cell derived cathepsin L. To sum up, fibroblasts can promote carcinoma cell dissemination by several different mechanisms. Extracellular matrix and basement membrane proteins provide attachment sites for cell locomotion promoting adhesion receptors. Growth factors and metalloproteinases are known to accelerate cell invasion. Additionally, cancer cell–fibroblast interplay generates biologically active fragments of basement membrane proteins, such as endostatin.

2020-05-04 | PXD016882 | Pride

Quantitative Analysis of negative control and overexpression-TRIB1 in PC3 and DU145 Transcriptomes

Project description:The goals of this study is to find effect of overexpression-TRIB1 to prostate cancer cell (PC3 and DU145).

2019-01-08 | GSE124733 | GEO

Effect of FGF19 and TNFalpha on human DU145 prostate cancer cells

Project description:DU145 prostate cancer cells were treated with 50 ng/ml FGF19 and 50 ug/ml heparin, or 10 ng/ml TNFalpha, or both

2010-07-09 | GSE22807 | GEO

Analysis of gene expression in sublines of DU145 human prostate cancer cells created through in vivo metastatic selection

Project description:DU145 human prostate cancer cells were injected into the prostate of nude mice and cells metastasizing to lymph node (LN1) grown and experiment repeated to make LN2-4. DU145 human prostate cancer cells were injected into the tail vein of nude mice and cells metastasizing to lung (ivLU1) grown and experiment repeated to make ivLU2-4.

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RNAseq of prostate cancer cells (PC3 and DU145) treated with 2.5 uM pentamidine or vehicle

Project description:To uncover the mechanism underlying the effect of pentamidine on prostate cancer cells, we performed RNA sequencing (RNA-seq) to compare the transcriptional difference between pentamidine- and vehicle-treated prostate cancer cells (PC3 and DU145 cells).

2019-06-14 | GSE132693 | GEO

Analysis of gene expression in sublines of DU145 human prostate cancer cells created through in vivo metastatic selection

2013-10-29 | E-GEOD-51755 | biostudies-arrayexpress

Effect of FGF19 and TNFalpha on human DU145 prostate cancer cells

Project description:DU145 prostate cancer cells were treated with 50 ng/ml FGF19 and 50 ug/ml heparin, or 10 ng/ml TNFalpha, or both drug treatment groups

2010-07-09 | E-GEOD-22807 | biostudies-arrayexpress

Genes modulated by miR-205 in DU145 prostate cancer cells

Project description:The study was aimed at identifying genes directly or indirectly regulated by miR-205 in the prostate. To this purpose, DU145 prostate cancer cells, which express miR-205 at very low levels, were transfected with miR-205 synthetic precursor and consequent alterations of gene expression analyzed using a microarray approach. Keywords: comparison betweed cells exposed to different miRNA precursors

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