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Analysis of gene expression in sublines of DU145 human prostate cancer cells created through in vivo metastatic selection

ABSTRACT: DU145 human prostate cancer cells were injected into the prostate of nude mice and cells metastasizing to lymph node (LN1) grown and experiment repeated to make LN2-4. DU145 human prostate cancer cells were injected into the tail vein of nude mice and cells metastasizing to lung (ivLU1) grown and experiment repeated to make ivLU2-4. 8 groups representing individual cell lines

ORGANISM(S): Homo sapiens

SUBMITTER: Jacqueline Banyard

PROVIDER: E-GEOD-51755 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Analysis of gene expression in sublines of DU145 human prostate cancer cells created through in vivo metastatic selection

Project description:DU145 human prostate cancer cells were injected into the prostate of nude mice and cells metastasizing to lymph node (LN1) grown and experiment repeated to make LN2-4. DU145 human prostate cancer cells were injected into the tail vein of nude mice and cells metastasizing to lung (ivLU1) grown and experiment repeated to make ivLU2-4.

2013-10-29 | GSE51755 | GEO

Gene and miRNA expression data in the DU145-LN metastatic cell series

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-10-29 | E-GEOD-51756 | biostudies-arrayexpress

Next generation RNA sequencing elucidates the antitumor effects of Zelnorm via immune microenvironment regulation using DU145 tumor model

Project description:Prostate cancer DU145 tumors were subcutaneously established in male nude mice. In nude mice, Zelnorm were orally administrated. Tumor volume and body weight were assessed (tumor volume=1/2 (length×width2)). Tumors were excised and weighted. Tumor samples were profiled by RNA-seq analysis. Respectively, to differentiate the gene expression originated from human (cancer cells) or mouse (tumor TME part), data were mapped against human (GRCh38) and mouse (GRCm38) genomes separately.

2023-12-31 | GSE198765 | GEO

Hedgehog/GLI signalling and hormone independence in prostate cancer (PCa) cell lines

Project description:High levels of GLI (GLI1 and GLI2) mRNA and GLI luciferase reporter activity were detected in the androgen independent prostate cancer cell lines DU145 and PC-3 compared to the androgen-dependent LNCaP prostate cancer cell line. Subsequently, we observed that ectopic GLI1 promoted hormone independence in LNCaP cells (LNCaP-GLI1). We compared the gene expression profile of LNCaP-pBP (empty vector), LNCaP-GLI1, DU145, and PC-3 cells globally as well as to identify GLI1-regulated genes that may contribute to hormone independence. RNA was harvested and analysed from LNCap-pBP (control/reference sample), LNCaP-GLI1, DU145 and PC-3 cells

2011-02-13 | E-GEOD-27231 | biostudies-arrayexpress

Expression data of HGF/cMET pathway in prostate cancer DU145 cell line

Project description:DU145 prostate cancer cells were treated with 25 ng/ml hepatocyte growth factor (HGF) or vehicle for 2, 8, or 24 hours. HGF stimulates the cMET protein, a tyrosine kinase transmembrane protein. The aim of this study is to determine the role of the HGF/cMET pathway in immature cells of established prostate cancer. HGF stimulation of DU145 prostate cancer cell line led to cell migration in culture, formation of sprouts in Matrigel and inhibition of growth. These biological effects went together with induction of a stem-like phenotype as defined by up-regulation of CD49b, CD49f, CD44 and SOX9, and down-regulation of CD24 on gene-expression arrays and quantitative PCR. The shift towards a stem-like phenotype was reflected by protein modifications on FACS, Western blot, and enhanced rapid adhesion to collagen I. Small molecules SU11274 and PHA665752 were able to inhibit both morphologic and molecular HGF effects. DU145 cells were stimulated for 2, 8 and 24 hours with 25 ng/ml HGF or vehicle. For each time point two arrays analyses were performed. One for cells stimulated with a vehicle and one for the HGF stimulated cells. Six arrays were performed in total in this study.

2011-12-16 | E-GEOD-16659 | biostudies-arrayexpress

Comparative analysis of gene expression of radioresistant prostate cancer cells and prostate cancer progenitors

Project description:By employing a global gene expression profiling, we performed analysis of the molecular pathways which are deregulated in DU145 ALDH+ prostate cancer progenitors and DU145-RR radioresistant cells. Non-sorted, ALDH-negative, and ALDH-positive populations were analyzed, both for parental DU145 cell line and for its radioresistant sub-line. Three independent experiments were performed

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SWATH proteomic profiling of prostate cancer cells identifies NUSAP1 as a biomarker of response to Galiellalactone

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2019-01-14 | PXD010993 | Pride

Low Dose PDT - Human Cells

Project description:Human tumour cell lines PC3, DU145, U87 and U373 (prostate carcinoma and glioblastoma) were treated with photosensitizers 5-aminolaevulinic acid (5-ALA) or photofrin. Then, the cells were irradiated sublethally with 635 nm laser light.<br>After photodynamic therapy, the cells were grown at 37°C for 4 or 24 hours in the dark until extraction of total RNA and expression profiling.

2011-06-26 | E-MEXP-3020 | biostudies-arrayexpress

Expression data of cancer cell lines

Project description:Expression profiles of aggressive versus non-aggressive ovarian, breast, melanoma, and prostate cancer cell lines Expression profiles of aggressive versus non-aggressive ovarian, breast, melanoma, and prostate cancer cell lines was determined. 231MFP, C8161, SKOV3, DU145, and PC3 are aggressive and MCF7, MUM2C, OVCAR3, and LNCaP are non-aggressive cancer cells. We are not comparing across all of the cell lines--just between C8161 and MUM2C, SKOV3 and OVCAR3, 231MFP and MCF7, and LNCaP/DU145/PC3. Therefore the normalization strategies used are different. We have not used the same normalization strategy

2011-08-09 | E-GEOD-28846 | biostudies-arrayexpress

Modulation of ST6Gal1 in Prostate Cancer Cells

Project description:Proteomics analyses using LC-MS/MS was performed on wild-type and ST6Gal1 overexpressing DU145 prostate cancer cells. Total cell lysate and secreted proteins from both were analyzed.

2023-11-06 | PXD041316 | Pride

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