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Comparison of glucocorticoid receptor (NR3C1) binding in wild type and Klf15-/- MEFs

ABSTRACT: The objective of this study was to dertemine GR binding patterns within wild type murine embryonic fibroblasts in comparison to embryonic fibroblasts derived from mice containing a null mutation for Klf15.

ORGANISM(S): Mus musculus

PROVIDER: GSE69947 | GEO | 2015/06/18

SECONDARY ACCESSION(S): PRJNA287225

REPOSITORIES: GEO

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Comparison of glucocorticoid receptor (NR3C1) binding in wild type and Klf15-/- MEFs

Project description:The objective of this study was to dertemine GR binding patterns within wild type murine embryonic fibroblasts in comparison to embryonic fibroblasts derived from mice containing a null mutation for Klf15. This study utilized duplicate samples and the following conditions. Wild type cells treated with ethanol (pooled single sample secondary to low starting material); wild type cells treated with dexamethasone for 1 hour (2 samples); Klf15-/- cells treated with ethanol (2 samples); Klf15 -/- cells treated with dexamethasone (2 samples). Samples treated with ethanol served as controls.

2015-06-18 | E-GEOD-69947 | biostudies-arrayexpress

Effects of dexamethasone and KLF15 on genome-wide occupancy of the glucocorticoid receptor (NR3C1) and RNA polymerase II (RNAPII) in human airway smooth muscle

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KLF15

Project description:KLF15

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Genome-wide transcriptional targets of KLF15 in human airway smooth muscle

Project description:We previously demonstrated that the transcription factor, KLF15, is a glucocorticoid-regulated gene that represses primary human airway smooth muscle (ASM) proliferation. Here, we show that KLF15 also represses ASM hypertrophy. To uncover the mechanistic basis for these effects, we integrated transcriptome data from KLF15 over-expression with genome-wide analysis of RNA Polymerase II (RNAPII) and glucocorticoid receptor (GR) occupancy (i.e. ChIP-seq). This led us to identify PLCD1 as both a KLF15-regulated gene and a repressor of ASM hypertrophy.

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Transcription profiling of mouse livers and quadriceps muscles of Kruppel-like factor 15 (KLF15) null mutants after an overnight fast to provide insight into the role of KLF15 in gluconeogenesis

Project description:We used microarray analysis to identify differences in gene expression levels, in liver and in quadriceps skeletal muscle, between 18h (overnight) fasted WT control and Kruppel-like factor 15 (KLF15)-null mice. Experiment Overall Design: Liver and skeletal muscle (quadriceps) tissues were isolated from 4-5 month old, 18h-fasted WT and KLF15-null female mice for RNA extraction and hybridization on Affymetrix microarrays.

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Essential functions of the Glucocorticoid Receptor require direct DNA binding [RNA-seq]

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2019-12-31 | GSE124919 | GEO

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